Xuan Ming, Fang Yi-Ru, Wato Masahiro, Hata Shintaro, Tanaka Akio
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, People's Republic of China.
J Oral Pathol Med. 2005 Jul;34(6):334-9. doi: 10.1111/j.1600-0714.2005.00316.x.
Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.
Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3'-diaminobenzidine (DAB) as color reagents, respectively.
In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.
These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral.
区分淋巴管和血管很困难，部分原因是缺乏识别淋巴管的特定方法。一种新的淋巴管反应性抗体D2-40最近已上市。我们研究了D2-40对口腔肿瘤性病变中淋巴管与血管鉴别的选择性。
采用ENVISION聚合物技术，分别以5-溴-4-氯-3-吲哚磷酸酯（BCIP）/氯化硝基四氮唑蓝（NBT）和3,3'-二氨基联苯胺（DAB）作为显色剂，对口腔淋巴管瘤（n = 3）、口腔血管瘤（n = 7）和口腔鳞状细胞癌（OSCC，n = 46）的福尔马林固定、石蜡包埋切片进行D2-40和抗CD34单克隆抗体（MoAb）双重免疫染色。
在口腔淋巴管瘤和血管瘤中，所有淋巴管均检测到D2-40，而所有血管CD34均呈阳性。在OSCC中，肿瘤周围区域淋巴管（P < 0.01）和血管数量明显多于肿瘤内区域。
这些结果表明，肿瘤周围区域的淋巴管增生可能比肿瘤内更为广泛。
