α-防御素-1在慢性增生性念珠菌病中的表达
Expression of alpha-defensin-1 in chronic hyperplastic candidosis.
作者信息
Ali Ahmed, Niissalo Sirkku, Hietanen Jarkko, Laine Mikael, Rautemaa Riina, Konttinen Yrjö
机构信息
Department of Anatomy/Biomedicum, University of Helsinki, Helsinki, Finland.
出版信息
J Oral Pathol Med. 2005 Jul;34(6):347-51. doi: 10.1111/j.1600-0714.2005.00317.x.
BACKGROUND
Chronic hyperplastic candidosis (CHC) represents a chronic opportunistic candida infection. We clarified the presence, localization and participation of alpha-defensin-1 in host response against chronic candidal stimulus.
METHODS
Immunohistochemically stained CHC biopsies (n = 10) were compared to candida negative idiopathic leukoplakia (n = 10).
RESULTS
In CHC alpha-defensin-1 was detected in neutrophils intravascularly, in lamina propria and in the epithelium, in part in intraepithelial microabscesses. Staining intensity of individual neutrophils varied and was associated with peri- and extracellular staining, in particular in the superficial epithelial cell layers. In controls only very few homogeneously staining neutrophils were detected intravascularly without any extracellular alpha-defensin-1 deposition.
CONCLUSIONS
Neutrophils form microabscesses and respond to Candida by activation and release of alpha-defensin-1 to peri- and extracellular matrix. This together with the epithelial cell migration from the basal layer to epithelial surface leads to alpha-defensin-1 rich protective shield in the most superficial epithelial cell layers.
背景
慢性增生性念珠菌病（CHC）是一种慢性机会性念珠菌感染。我们阐明了α-防御素-1在宿主对慢性念珠菌刺激的反应中的存在、定位及作用。
方法
将免疫组织化学染色的CHC活检标本（n = 10）与念珠菌阴性的特发性白斑（n = 10）进行比较。
结果
在CHC中，α-防御素-1在血管内的中性粒细胞、固有层和上皮中均有检测到，部分存在于上皮内微脓肿中。单个中性粒细胞的染色强度各不相同，且与周围和细胞外染色有关，尤其是在上皮浅层细胞层。在对照组中，仅在血管内检测到极少数均匀染色的中性粒细胞，无任何细胞外α-防御素-1沉积。
结论
中性粒细胞形成微脓肿，并通过激活和释放α-防御素-1至周围和细胞外基质来应对念珠菌。这与上皮细胞从基底层迁移至上皮表面一起，在上皮最浅层细胞层形成富含α-防御素-1的保护屏障。
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