带绦虫属：用于分子系统诊断的18S核糖体DNA微卫星

Taenia spp.: 18S rDNA microsatellites for molecular systematic diagnosis.

作者信息

Foronda P, Casanova J C, Martinez E, Valladares B, Feliu C

机构信息

Department of Parasitology, Ecology and Genetics, Faculty of Pharmacy, University of La Laguna, Tenerife, Canary Islands, Spain.

出版信息

J Helminthol. 2005 Jun;79(2):139-42. doi: 10.1079/joh2005280.

DOI:10.1079/joh2005280

PMID:15946391

Abstract

The 18S rDNA gene of adult worms of Taenia parva found in Genetta genetta in the Iberian Peninsula and larval stages of T. pisiformis from the wild rabbit (Oryctolagus cuniculus) in Tenerife (Canary Islands) were amplified and sequenced. The sequences of the 18S rDNA gene of T. parva (1768 bp) and T. pisiformis (1760 bp) are reported for the first time (GenBank accession nos. AJ555167-AJ555168 and AJ555169-AJ555170, respectively). In 168 alignment positions microsatellites in the 18S rDNA of both taxa were detected for the first time (TGC in T. parva and TGCT in T. pisiformis) and differences in their sequences with different repetition numbers were observed. The use of nucleotide sequences of this gene in the resolution of systematic problems in cestodes is discussed with reference to the systematic status of Taenia spp. and mainly in human taeniids such as T. solium, T. saginata, and Asian human isolates of Taenia.

摘要

对在伊比利亚半岛的灵猫属动物中发现的微小膜壳绦虫成虫的18S rDNA基因以及来自特内里费岛（加那利群岛）野生兔（穴兔）的豆状带绦虫幼虫阶段进行了扩增和测序。首次报道了微小膜壳绦虫（1768 bp）和豆状带绦虫（1760 bp）的18S rDNA基因序列（GenBank登录号分别为AJ555167 - AJ555168和AJ555169 - AJ555170）。首次在两个分类单元的18S rDNA的168个比对位置检测到微卫星（微小膜壳绦虫为TGC，豆状带绦虫为TGCT），并观察到它们具有不同重复数的序列差异。参考绦虫属的系统地位，主要是在诸如猪带绦虫、牛带绦虫和亚洲人体分离的带绦虫等人体带绦虫方面，讨论了该基因的核苷酸序列在解决绦虫系统发育问题中的应用。