Cui Xiao-nan, Tang Jian-wu, Hou Li, Song Bo, Liu Ji-wei
Department of Oncology, the 1st Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
Zhonghua Zhong Liu Za Zhi. 2005 Mar;27(3):138-40.
To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastasis Hca-F and its synogenetic cell line Hca-P with low metastatic potential was constructed by suppression subtractive hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.
Ten differentially expressed cDNA fragments of Hca-F with high potential of lymphatic spreading were obtained, two of which were newly identified ones.
SSH is a useful technique to detect genes of differential expression and an effective method to clone novel genes.
筛选在具有高淋巴转移潜能的小鼠肝癌腹水细胞系中差异表达的基因。
采用抑制性消减杂交（SSH）法构建具有高淋巴转移潜能的小鼠肝癌细胞系Hca-F及其低转移潜能的同源细胞系Hca-P的消减cDNA文库。对筛选出的消减文库克隆进行测序，并进行GenBank同源性搜索。
获得了10个具有高淋巴转移潜能的Hca-F差异表达cDNA片段，其中2个为新鉴定的片段。
SSH是检测差异表达基因的有用技术，也是克隆新基因的有效方法。
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