低淋巴道转移潜能小鼠肝癌腹水细胞系中差异表达基因的鉴定
Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis.
作者信息
Cui Xiao-Nan, Tang Jian-Wu, Hou Li, Song Bo, Ban Li-Ying
机构信息
Department of Oncology, The 1st Affiliated Dalian Medical University, Dalian 116027, Liaoning Province, China.
出版信息
World J Gastroenterol. 2006 Nov 14;12(42):6893-7. doi: 10.3748/wjg.v12.i42.6893.
AIM
To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis.
METHODS
A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lympho-genous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.
RESULTS
Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes, 4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology.
CONCLUSION
Tumor metastasis is an incident involving multiple genes. SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.
目的
鉴定在低淋巴道转移潜能的小鼠肝癌腹水细胞系中差异表达的基因。
方法
采用抑制性消减杂交（SSH）法构建低淋巴道转移潜能的小鼠肝癌细胞系Hca-P及其高转移潜能的同基因细胞系Hca-F的消减cDNA文库。对筛选出的消减文库克隆进行测序，并进行GenBank同源性搜索。
结果
获得了15个Hca-P差异表达的cDNA片段，其中8个为已知基因，4个为表达序列标签（EST），3个cDNA无同源性。
结论
肿瘤转移是一个涉及多个基因的事件。SSH是检测差异表达基因的有用技术，也是克隆新基因的有效方法。
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