[骨桥蛋白在胃癌中的表达及其与侵袭和转移的关系]

[Osteopontin expression and its relation to invasion and metastases in gastric cancer].

作者信息

Zhang Dong-tao, Yuan Jing, Yang Li, Guo Xin-ning, Hao Zhi-ming, Han Zhe-yi, Wu Kai-chun, Fan Dai-ming

机构信息

The Key Laboratory of Bio-technology, Life Science School of Ningxia University, Yinchuan 750021, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2005 Mar;27(3):167-9.

PMID:15946568

Abstract

OBJECTIVE

To investigate the correlation between expression of the osteopontin (OPN) and invasion and metastases in gastric cancer.

METHODS

The expression of OPN, NF-kappaB p65 and matrix metallo-proteinase 9 (MMP-9) was detected by immunohistochemistry in non-cancer gastric tissue (n = 12 cases) and gastric cancer tissue (n = 72 cases).

RESULTS

(1) OPN, NF-kappaB p65 and MMP-9 were not expressed in 12 non-cancer gastric tissue samples(group A). Their expression rates were 43.3%, 40.0% and 46.7% respectively in 30 gastric cancer samples without lymph nodes metastasis (group B), but they increased to 76.9%, 73.1% and 80.8% in 26 gastric cancer samples with lymph nodes metastases (group C), and 87.5%, 81.3% and 93.8% respectively in 16 gastric cancer samples with lymph node and distant metastases (group D). (2) There were statistically significant differences in their expressions between group D and group B (P(a) = 0.004, P(c) = 0.007, P(e) = 0.002), and between group C and group B (P(b) = 0.011, P(d) = 0.013, P(f) = 0.009). (3) Despite some differences in positive expression rates, correlations existed between OPN and NF-kappaB p65, and between NF-kappaB p65 and MMP-9 (P(1) = 0.042, P(2) = 0.013; r(1)= 0.67, r(2)= 0.72).

CONCLUSION

Osteopondin espression is closely related to the invasion and metastases of gastric cancer. It may upregulate the expression of metastasis-related molecule MMP-9 by activating NF-kappaB pathway.

摘要

目的

探讨骨桥蛋白（OPN）表达与胃癌侵袭和转移之间的相关性。

方法

采用免疫组织化学法检测12例非癌胃组织和72例胃癌组织中OPN、核因子κB p65（NF-κB p65）和基质金属蛋白酶9（MMP-9）的表达。

结果

（1）12例非癌胃组织样本（A组）中未检测到OPN、NF-κB p65和MMP-9表达。30例无淋巴结转移的胃癌样本（B组）中它们的表达率分别为43.3%、40.0%和46.7%，而在26例有淋巴结转移的胃癌样本（C组）中它们分别增至76.9%、73.1%和80.8%，在16例有淋巴结及远处转移的胃癌样本（D组）中分别为87.5%、81.3%和93.8%。（2）D组与B组之间以及C组与B组之间它们的表达存在统计学差异（P(a)=0.004，P(c)=0.007，P(e)=0.002；P(b)=0.011，P(d)=0.013，P(f)=0.009）。（3）尽管阳性表达率存在一些差异，但OPN与NF-κB p65之间以及NF-κB p65与MMP-9之间存在相关性（P(1)=0.042，P(2)=0.013；r(1)=0.67，r(2)=0.72）。