小鼠视网膜色素上皮/脉络膜中基因空间表达随衰老的变化。
Changes in the spatial expression of genes with aging in the mouse RPE/choroid.
作者信息
Ogawa Tsukihiko, Boylan Sharon A, Oltjen Sharon L, Hjelmeland Leonard M
机构信息
Department of Biological Chemistry, School of Medicine, University of California, Davis, CA 95616-8794, USA.
出版信息
Mol Vis. 2005 Jun 1;11:380-6.
PURPOSE
We recently used microarray and reverse transcriptase PCR (RT-PCR) analysis to show an upregulation of cathepsin S (CatS) and glutathione peroxidase 3 (GPX3) in the aging mouse RPE/choroid. To evaluate the mRNA distribution and levels in the RPE and choroid, in situ hybridizations were performed.
METHODS
Eye sections from 2-month-old and 24-month-old C57BL/6 mice were probed for CatS or GPX3 mRNA by in situ hybridization. The ratio of mRNA labeled cells to total cells counted per section was compared between the two age groups for the RPE and choroid separately.
RESULTS
The CatS labeled RPE cell ratio increased significantly with age. The GPX3 labeled RPE cell ratio did not increase with age.
CONCLUSIONS
The increases in mRNA levels for CatS and GPX3 found in the aging C57BL/6 RPE/choroid appear to represent an increase in both the numbers of cells expressing these messages and an increase in the level of expression in individual cells.
目的
我们最近使用微阵列和逆转录聚合酶链反应（RT-PCR）分析显示，衰老小鼠视网膜色素上皮/脉络膜中组织蛋白酶S（CatS）和谷胱甘肽过氧化物酶3（GPX3）上调。为了评估视网膜色素上皮（RPE）和脉络膜中mRNA的分布和水平，我们进行了原位杂交。
方法
通过原位杂交检测2月龄和24月龄C57BL/6小鼠眼组织切片中CatS或GPX3 mRNA。分别比较两个年龄组RPE和脉络膜每切片中mRNA标记细胞与总细胞计数的比例。
结果
CatS标记的RPE细胞比例随年龄显著增加。GPX3标记的RPE细胞比例不随年龄增加。
结论
在衰老的C57BL/6 RPE/脉络膜中发现的CatS和GPX3 mRNA水平的增加似乎代表了表达这些信息的细胞数量增加以及单个细胞中表达水平的增加。
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