N4,N9-二油酰精胺是一种用于质粒DNA制剂的新型非病毒脂质多胺载体。
N4,N9-dioleoyl spermine is a novel nonviral lipopolyamine vector for plasmid DNA formulation.
作者信息
Ahmed Osama A A, Adjimatera Noppadon, Pourzand Charareh, Blagbrough Ian S
机构信息
Department of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, United Kingdom.
出版信息
Pharm Res. 2005 Jun;22(6):972-80. doi: 10.1007/s11095-005-4592-1. Epub 2005 Jun 8.
PURPOSE
To study the effect of synthesized N4,N9-dioleoyl spermine on DNA condensation and then measure its transfection efficiency in cell culture.
METHODS
The lipopolyamine was synthesized from the naturally occurring polyamine spermine. The ability of this novel compound to condense DNA was studied using ethidium bromide fluorescence quenching and light scattering assays. Transfection efficiency was studied in primary skin cells (FEK4) and in an immortalized cancer cell line (HtTA), and compared with the commercially available transfection formulations Lipofectin and Lipofectamine.
RESULTS
The synthesized N4,N9-dioleoyl spermine formula is efficient at condensing calf thymus and circular plasmid DNA and effectively transfects both primary skin cells and cancer cell lines at low charge ratios of (+/- ammonium/phosphate) 2.5.
CONCLUSIONS
N4,N9-Dioleoyl spermine condenses DNA and achieves high transfection levels in cultured cells.
目的
研究合成的N4,N9 - 二油酰精胺对DNA凝聚的影响，然后在细胞培养中测定其转染效率。
方法
从天然存在的多胺精胺合成脂多胺。使用溴化乙锭荧光猝灭和光散射测定法研究这种新型化合物凝聚DNA的能力。在原代皮肤细胞（FEK4）和永生化癌细胞系（HtTA）中研究转染效率，并与市售转染制剂Lipofectin和Lipofectamine进行比较。
结果
合成的N4,N9 - 二油酰精胺配方在凝聚小牛胸腺和环状质粒DNA方面有效，并且在（+/-铵/磷酸盐）2.5的低电荷比下能有效转染原代皮肤细胞和癌细胞系。
结论
N4,N9 - 二油酰精胺凝聚DNA并在培养细胞中实现高转染水平。
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