Fleisher Helen C, Vigh Gyula
Department of Chemistry, Texas A&M University, College Station, TX 77842-3012, USA.
Electrophoresis. 2005 Jun;26(13):2511-9. doi: 10.1002/elps.200500198.
Diaminocarboxylic acid carrier ampholytes, such as L-histidine, 2,3-diaminopropionic acid, L-ornithine, and L-lysine, were reacted with glycerol-1,3-diglycidyl ether (GDGE) and poly(vinyl alcohol) (PVA) in the presence of sodium hydroxide to produce hydrolytically and mechanically stable hydrogels, supported on a PVA substrate, for use as buffering membranes in isoelectric trapping (IET) separations. The pH values of the DACAPVA membranes were determined with the help of small-molecule pI markers and proteins and were found to be in the 6 < pH < 8.5 range. The membranes were successfully used to isoelectrically trap small ampholytes, desalt ampholyte solutions in IET mode, and effect the binary separation of chicken egg white proteins.
二氨基羧酸载体两性电解质，如L-组氨酸、2,3-二氨基丙酸、L-鸟氨酸和L-赖氨酸，在氢氧化钠存在下与甘油-1,3-二缩水甘油醚（GDGE）和聚乙烯醇（PVA）反应，以制备负载在PVA基质上的水解稳定且机械稳定的水凝胶，用作等电捕集（IET）分离中的缓冲膜。借助小分子pI标记物和蛋白质测定了DACAPVA膜的pH值，发现其在6＜pH＜8.5范围内。这些膜成功用于等电捕集小两性电解质、在IET模式下对两性电解质溶液进行脱盐以及实现蛋清蛋白的二元分离。
