Ogier Agathe, Franco Manuel A, Charpilienne Annie, Cohen Jean, Pothier Pierre, Kohli Evelyne
Microbiologie Médicale et Moléculaire, EA562, UFRs Médecine et Pharmacie, Laboratoire de Virologie CHU, Dijon, France.
Eur J Immunol. 2005 Jul;35(7):2122-30. doi: 10.1002/eji.200526059.
Virus-like particles containing the rotavirus (RV) internal proteins VP2 and VP6 (2/6-VLP) have been shown to induce serum and fecal antibodies as well as protection in mice after intranasal administration with a mutant of E. coli toxin, LT-R192G. To better understand the origin of fecal IgA induced by this protocol, we studied the RV-specific B cell response in systemic and mucosal lymphoid tissues using a flow cytometry assay that allows quantification and phenotypic characterization of RV-specific B lymphocytes. We also assessed the RV-specific antibody-secreting cells in the spleen and intestinal lamina propria (ILP). A remarkably high frequency of RV-specific B cells was found in the respiratory lymphoid tissues and spleen, of which only a minority expressed the alpha4beta7 integrin (intestinal homing receptor). In contrast, but in accordance with alpha4beta7 expression at the induction site, a very low response was observed in intestinal lymphoid tissues (mesenteric lymph nodes and ILP), which did not increase after a second immunization. Thus, intranasal immunization with a nonreplicating antigen does not induce an important number of RV-specific B cells with an intestinal homing profile.
含有轮状病毒（RV）内部蛋白VP2和VP6的病毒样颗粒（2/6-VLP）已被证明，在用大肠杆菌毒素突变体LT-R192G经鼻给药后，可在小鼠体内诱导血清和粪便抗体以及产生保护作用。为了更好地理解该方案诱导的粪便IgA的来源，我们使用流式细胞术检测方法研究了全身和黏膜淋巴组织中RV特异性B细胞反应，该方法可对RV特异性B淋巴细胞进行定量和表型特征分析。我们还评估了脾脏和肠固有层（ILP）中RV特异性抗体分泌细胞。在呼吸道淋巴组织和脾脏中发现了频率极高的RV特异性B细胞，其中只有少数细胞表达α4β7整合素（肠道归巢受体）。相比之下，与诱导部位的α4β7表达情况一致，在肠道淋巴组织（肠系膜淋巴结和ILP）中观察到的反应非常低，二次免疫后也没有增加。因此，用非复制性抗原进行鼻内免疫不会诱导大量具有肠道归巢特征的RV特异性B细胞。
