Plessl Markus, Rigola Diana, Hassinen Viivi, Aarts Mark G M, Schat Henk
Institute of Biochemical Plant Pathology, GSF, National Research Center for Environment and Health, Ingolstaedter Landdstr, Germany.
Z Naturforsch C J Biosci. 2005 Mar-Apr;60(3-4):216-23. doi: 10.1515/znc-2005-3-406.
Thlaspi caerulescens is a well-studied metal-hyperaccumulator of zinc, cadmium and nickel, belonging to the Brassicaceae family. Moreover it is one of the few hyperaccumulators that occur on different metalliferous soil types, as well as on nonmetalliferous soils. We are interested in the development of systems to improve phytoremediation of metal contaminated soils through improved metal-accumulation. About 1900 cDNAs isolated from T. caerulescens roots were hybridized with reverse transcribed RNA from zinc-treated T. caerulescens plants of two accessions originating from two different soil types. This comparative transcript profiling of T. caerulescens plants resulted in the identification of genes that are affected by heavy metals. The developed microarray proved to be an appropriate tool for a large scale analysis of gene expression in this metal-accumulator species.
天蓝遏蓝菜是一种经过充分研究的锌、镉和镍超积累植物，属于十字花科。此外，它是少数能生长在不同金属土壤类型以及非金属土壤上的超积累植物之一。我们感兴趣的是开发通过提高金属积累来改善金属污染土壤植物修复的系统。从天蓝遏蓝菜根部分离出的约1900个cDNA与来自两种不同土壤类型的两个种质的经锌处理的天蓝遏蓝菜植物的逆转录RNA进行杂交。这种对天蓝遏蓝菜植物的比较转录谱分析导致了受重金属影响的基因的鉴定。所开发的微阵列被证明是对这种金属积累物种的基因表达进行大规模分析的合适工具。
