扩展基因组位点中DNA的组织特异性CpG甲基化研究。

Study of tissue-specific CpG methylation of DNA in extended genomic loci.

作者信息

Azhikina T L, Sverdlov E D

机构信息

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.

出版信息

Biochemistry (Mosc). 2005 May;70(5):596-603. doi: 10.1007/s10541-005-0153-9.

DOI:10.1007/s10541-005-0153-9

PMID:15948713

Abstract

Modern approaches for studies on genome functioning include investigation of its epigenetic regulation. Methylation of cytosines in CpG dinucleotides is an inherited epigenetic modification that is responsible for both functional activity of certain genomic loci and total chromosomal stability. This review describes the main approaches for studies on DNA methylation. Under consideration are site-specific approaches based on bisulfite sequencing and methyl-sensitive PCR, whole-genome approaches aimed at searching for new methylation hot spots, and also mapping of unmethylated CpG sites in extended genomic loci.

摘要

现代基因组功能研究方法包括对其表观遗传调控的研究。CpG二核苷酸中胞嘧啶的甲基化是一种可遗传的表观遗传修饰，它既负责某些基因组位点的功能活性，也负责整个染色体的稳定性。本综述描述了DNA甲基化研究的主要方法。所考虑的方法包括基于亚硫酸氢盐测序和甲基敏感PCR的位点特异性方法、旨在寻找新甲基化热点的全基因组方法，以及扩展基因组位点中未甲基化CpG位点的图谱绘制。