Li Hou-huai, Shen Zhen-ya, Hui Jie, Li Ke, Zheng Lin, Jiao Peng, Teng Xiao-mei, Zhu Jian-zhong, Gao Wei-dong, Yang Jun-hua, Zhou Bing-yuan
Department of Cardiovascular Surgery, the First Affiliated Hospital, Soochow University, Suzhou 215006, China.
Zhonghua Yi Xue Za Zhi. 2005 Mar 9;85(9):599-601.
We produced a large-animal model of myocardial infarction induced by transcatheter embolization of the left coronary artery using a gelatin sponge.
Seven pigs underwent transcatheter embolization of the left anterior descending artery (LAD) using gelatin sponge to produce anteroapical myocardial infarction (MI). 4 weeks later, Echocardiography, Coronary angiography and Pathology was performed, and the data were compared with those of the control group (n = 6).
The procedure mortality was 1 of 7. In the MI group, the LV end-diastolic dimension increased (control versus MI: 37.0 mm +/- 3.4 mm and 50.8 mm +/- 6.1 mm, P < 0.01), the ejection fraction (EF) decreased (control versus MI: 62.3% +/- 2.9% and 36.6% +/- 2.1%, P < 0.001). Coronary angiography revealed the LAD remained occluded. The postmortem specimen showed a transmural MI scar in the anteroseptal and apical regions in the MI group and the LV volumes at 30 mm Hg were 81.4 ml +/- 4.3 ml, the scar areas were 18.4% +/- 1.6% of total left ventricular free wall and the scar thickness was 3.5 mm +/- 0.8 mm. Histologic samples in the MI group stained with Masson's trichrome showed massive fibrosis in the border zone and patchy fibrosis in the remote region in the LV free wall, whereas the control group showed no fibrosis.
This pig model of myocardial infarction is reliable, reproducible, and similar to the human condition, amenable to investigate other investigation.
我们使用明胶海绵通过经导管栓塞左冠状动脉制作了一种大型动物心肌梗死模型。
7头猪使用明胶海绵对左前降支动脉（LAD）进行经导管栓塞以产生前壁心尖部心肌梗死（MI）。4周后，进行超声心动图、冠状动脉造影和病理学检查，并将数据与对照组（n = 6）的数据进行比较。
手术死亡率为7头中的1头。在MI组中，左心室舒张末期内径增加（对照组与MI组：37.0 mm±3.4 mm和50.8 mm±6.1 mm，P < 0.01），射血分数（EF）降低（对照组与MI组：62.3%±2.9%和36.6%±2.1%，P < 0.001）。冠状动脉造影显示LAD仍闭塞。尸检标本显示MI组在前间隔和心尖区域有透壁性MI瘢痕，30 mmHg时左心室容积为81.4 ml±4.3 ml，瘢痕面积占左心室游离壁总面积的18.4%±1.6%，瘢痕厚度为3.5 mm±0.8 mm。MI组用Masson三色染色的组织学样本显示左心室游离壁边缘区有大量纤维化，远隔区域有散在纤维化，而对照组未显示纤维化。
这种猪心肌梗死模型可靠、可重复，且与人类情况相似，适合进行其他研究。
