Qiao Chong, Cheng Da-Li, Zhang Shu-Lan, Wang Chun-Hui, Lin Qi-de
Department of Obstetrics and Gynecology, Second Affiliated Hospital of China Medical University, Shenyang 110004, China.
Zhonghua Yi Xue Za Zhi. 2005 Mar 30;85(12):839-42.
To explore the role of metastasis-related gene KiSS-1 and matrix metalloproteinase (MMP)-9 in regulation of invasion of trophoblasts.
RT-PCR and Western blotting were used to detect the MMP-9 and KiSS-1 expression levels in the placental tissues obtained from 90 cases of normal pregnant women undergoing artificial abortion, induction of labor with water bag or selective cesarean section among which 30 cases were in the first trimester, 30 in second-trimester and 30 cases of term pregnancy, and in the placental tissues of 40 cases of preeclampsia (15 cases of mild and 25 cases of severe preeclampsia) undergoing cesarean section, and tissues of 90 cases of hydatidiform mole, 9 cases of invasive mole and 8 cases of choriocarcinoma, all undergoing surgery.
The expression levels of MMP-9 mRNA and protein were higher in first-trimester [A value 0.391 +/- 0.215, (36 +/- 7) microg/100 microg total protein] and then decreased gradually with the progress of gestation. The expression levels of MMP-9mRNA and protein in the term placental samples were significantly lower than those of first-trimester (both P < 0.01). The expression levels of KiSS-1mRNA and metastin in normal placenta increased along with the progress of gestation (both P < 0.01). The KISS-1 mRNA expression level and MMP-9 protein expression level in the placental tissue of preeclampsia were 0.09 +/- 0.06 (A value) and (9.6 +/- 4.3) microg/100 microg total protein respectively, both significantly lower than those of the term placenta (both P < 0.01). The expression levels of MMP-9 mRNA and protein in the tissues of gestational trophoblastic disease were significantly higher than those in the first-trimester placenta (both P < 0.01). The expression level of KISS-1 mRNA and metastin in the tissues of hyddatidiform mole and invasive mole were both significantly lower than those in the first trimester placenta (P < 0.05, P < 0.01). The expression of KiSS-1mRNA and metastin in the tissues of choriocarcinoma could not be detected.
The expression of the invasion-related gene, MMP-9, is positively related with, while the invasion suppressor gene, KiSS-1, is negatively related with the invasive ability of trophoblasts. The interaction of these two genes plays an important role in regulation of the invasion of trophoblasts.
探讨转移相关基因KiSS-1和基质金属蛋白酶（MMP）-9在滋养细胞侵袭调控中的作用。
采用逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法检测90例接受人工流产、水囊引产或选择性剖宫产的正常孕妇胎盘组织中MMP-9和KiSS-1的表达水平，其中早孕30例、中孕30例、足月妊娠30例；检测40例接受剖宫产的子痫前期患者（轻度15例、重度25例）胎盘组织中MMP-9和KiSS-1的表达水平；检测90例葡萄胎、9例侵蚀性葡萄胎和8例绒毛膜癌手术切除组织中MMP-9和KiSS-1的表达水平。
MMP-9 mRNA和蛋白表达水平在早孕时较高[A值为0.391±0.215，（36±7）μg/100μg总蛋白]，随后随孕周进展逐渐降低。足月胎盘样本中MMP-9 mRNA和蛋白表达水平显著低于早孕时（均P<0.01）。正常胎盘组织中KiSS-1 mRNA和metastin的表达随孕周进展而升高（均P<0.01）。子痫前期胎盘组织中KISS-1 mRNA表达水平和MMP-9蛋白表达水平分别为0.09±0.06（A值）和（9.6±4.3）μg/100μg总蛋白，均显著低于足月胎盘（均P<0.01）。妊娠滋养细胞疾病组织中MMP-9 mRNA和蛋白表达水平显著高于早孕胎盘组织（均P<0.01）。葡萄胎和侵蚀性葡萄胎组织中KISS-1 mRNA和metastin表达水平均显著低于早孕胎盘组织（P<0.05，P<0.01）。绒毛膜癌组织中未检测到KiSS-1 mRNA和metastin表达。
侵袭相关基因MMP-9的表达与滋养细胞侵袭能力呈正相关，而侵袭抑制基因KiSS-1的表达与滋养细胞侵袭能力呈负相关。这两个基因的相互作用在滋养细胞侵袭调控中起重要作用。
