Tsai Annabel T, Rice Julie, Scatena Marta, Liaw Lucy, Ratner Buddy D, Giachelli Cecilia M
Department of Bioengineering, University of Washington, Box 351720, Seattle, WA 98195, USA.
Biomaterials. 2005 Oct;26(29):5835-43. doi: 10.1016/j.biomaterials.2005.03.003. Epub 2005 Apr 21.
Foreign body giant cells (FBGCs) are a hallmark of the foreign body reaction caused by biomaterial implantation and are thought to contribute to biomaterial degradation and the duration of the response. Osteopontin (OPN) is a secreted, acidic matricellular protein with multiple phosphorylation sites that is highly expressed at sites of inflammation. OPN wildtype and knockout mice were implanted with poly(vinyl alcohol) sponges and explanted at 14 days. OPN knockout mice had more foreign body giant cells but fewer macrophages surrounding the implants than their wildtype counterparts. In an in vitro human FBGC assay, addition of soluble OPN was found to reduce macrophage fusion to giant cells. These are the first studies to show a direct inhibitory role of OPN in FBGC formation in response to implantation.
异物巨细胞（FBGCs）是生物材料植入引起的异物反应的标志，被认为有助于生物材料的降解和反应的持续时间。骨桥蛋白（OPN）是一种具有多个磷酸化位点的分泌型酸性基质细胞蛋白，在炎症部位高度表达。将OPN野生型和基因敲除小鼠植入聚乙烯醇海绵，并在14天后取出。与野生型小鼠相比，OPN基因敲除小鼠植入物周围的异物巨细胞更多，但巨噬细胞更少。在一项体外人FBGC检测中，发现添加可溶性OPN可减少巨噬细胞融合为巨细胞。这些是首次表明OPN在植入反应中对FBGC形成具有直接抑制作用的研究。
