肾上腺髓质素诱导大鼠血管平滑肌细胞中血红素加氧酶-1基因表达和环磷酸鸟苷生成。

Adrenomedullin induces heme oxygenase-1 gene expression and cGMP formation in rat vascular smooth muscle cells.

作者信息

Qi Yong-Fen, Dong Lin-Wang, Pan Chun-Shui, Zhang Jing, Geng Bin, Zhao Jing, Tang Chao-Shu

机构信息

Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034, PR China.

出版信息

Peptides. 2005 Jul;26(7):1257-63. doi: 10.1016/j.peptides.2005.01.012. Epub 2005 Feb 12.

DOI:10.1016/j.peptides.2005.01.012

PMID:15949644

Abstract

Adrenomedullin (ADM) is a potent vasodilatory peptide. It regulates blood pressure by increasing cyclic adenosine monophosphate (cAMP) and guanosine-3',5'-monophosphate (cGMP). We sought to investigate the effect of ADM on heme oxygenase-1 (HO-1) gene expression and cGMP formation in cultured rat vascular smooth muscle cells (VSMCs). ADM treatment, 10(-9) and 10(-8) mol/L, increased cGMP production, and it increased the intracellular cGMP content of platelets coincubated with VSMCs. It increased cGMP content by 158.8% and 273.5%, respectively; increased HO-1 activity by 49.5% and 87%, respectively; augmented HO-1 protein levels by 66% and 126%, respectively; upregulated the steady-state level of HO-1 mRNA by 73% and 159%, respectively, and increased HO-1 mRNA transcription synthesis by four- and seven-fold, respectively. These results suggest that ADM induces HO-1 gene expression and cGMP formation in rat VSMCs.

摘要

肾上腺髓质素（ADM）是一种强效血管舒张肽。它通过增加环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）来调节血压。我们试图研究ADM对培养的大鼠血管平滑肌细胞（VSMC）中血红素加氧酶-1（HO-1）基因表达和cGMP形成的影响。10^(-9)和10^(-8) mol/L的ADM处理可增加cGMP的产生，并增加与VSMC共同孵育的血小板的细胞内cGMP含量。它分别使cGMP含量增加了158.8%和273.5%；分别使HO-1活性增加了49.5%和87%；分别使HO-1蛋白水平增加了66%和126%；分别使HO-1 mRNA的稳态水平上调了73%和159%，并分别使HO-1 mRNA转录合成增加了4倍和7倍。这些结果表明，ADM可诱导大鼠VSMC中HO-1基因表达和cGMP形成。