Jeram S, Sintes J M Riego, Halder M, Fentanes J Baraibar, Sokull-Klüttgen B, Hutchinson T H
European Centre for the Validation of Alternative Methods (ECVAM), Institute for Health and Consumer Protection, Joint Research Centre, European Commission, 21020 Ispra (VA), Italy.
Regul Toxicol Pharmacol. 2005 Jul;42(2):218-24. doi: 10.1016/j.yrtph.2005.04.005.
This study explores the applicability of a fish acute threshold (step-down) test approach for the assessment of new chemical substances notified in the EU. The proposed approach basically implies replacing the fish LC50 toxicity test with a simple acute threshold test and thus reducing the number of fish used and also costs. The fish test would be performed only at one concentration, the lowest between the EC50 concentrations obtained with previous testing with algae and daphnia. When fish would be more sensitive than algae and daphnia, testing with fish would be continued at lower concentrations (step-down). From step-down test results the LC50 value can be obtained by applying the binominal method of interpolation. These data can be used together with algal and daphnid data to provide the same Predicted No Effect Concentration values. The acute aquatic toxicity data used in this evaluation were extracted from the New Chemicals Database of the European Chemicals Bureau. The results show that 53.6-71.2% reduction of the number of fish used would be possible when applying this new testing strategy and suggest its use for regulatory purposes.
本研究探讨了鱼类急性阈值（逐步降低）测试方法在评估欧盟通报的新化学物质方面的适用性。所提议的方法基本上意味着用一种简单的急性阈值测试取代鱼类LC50毒性测试，从而减少所用鱼类的数量以及成本。鱼类测试仅在一个浓度下进行，该浓度是先前用藻类和水蚤测试获得的EC50浓度中的最低值。当鱼类比藻类和水蚤更敏感时，将在更低浓度下继续进行鱼类测试（逐步降低）。从逐步降低测试结果中，可以通过应用二项式插值法获得LC50值。这些数据可与藻类和水蚤的数据一起用于提供相同的预测无效应浓度值。本评估中使用的急性水生毒性数据取自欧洲化学品管理局的新化学品数据库。结果表明，应用这种新的测试策略时，所用鱼类数量可能减少53.6%至71.2%，并建议将其用于监管目的。
