Causey Robert C, Weber James A, Emmans Erin E, Stephenson Lisa A, Homola Alma D, Knapp Kiley R, Crowley Ian F, Pelletier Danielle C, Wooley Nicole A
Department of Animal and Veterinary Sciences and the Maine Agriculture and Forestry Experiment Station, University of Maine, Orono, ME 04469-5735, USA.
Vet J. 2006 Sep;172(2):248-57. doi: 10.1016/j.tvjl.2005.03.014. Epub 2005 Jun 13.
The purpose of this study was to describe strain-specific immune responses to Streptococcus equi subspecies zooepidemicus (S. zooepidemicus) during uterine infection in horses. Five isolates of S. zooepidemicus were differentiated into four strains antigenically by bactericidal testing in blood of 12 horses, and genetically by pulsed-field gel electrophoresis. Eight healthy mares were then divided into two groups, each inoculated with one strain intrauterinely on three successive oestrous cycles followed by a second strain for three successive cycles, first and second strains being reversed for each group. Immune responses to both strains were assessed by bactericidal testing and immunoblotting over eight cycles. Both techniques indicated that immune responses to each strain arose at different times. Immunoblots showed greater binding to the first inoculated strain than to the second (P < 0.05). These data confirm that immune responses to S. zooepidemicus during uterine infection are partly strain-specific.
本研究的目的是描述马子宫感染期间针对马链球菌兽疫亚种（兽疫链球菌）的菌株特异性免疫反应。通过在12匹马的血液中进行杀菌试验，将5株兽疫链球菌在抗原性上分为4个菌株，并通过脉冲场凝胶电泳进行基因分型。然后将8匹健康母马分为两组，每组在三个连续发情周期内子宫内接种一种菌株，随后在三个连续周期内接种第二种菌株，每组的第一种和第二种菌株顺序相反。通过在八个周期内进行杀菌试验和免疫印迹评估对两种菌株的免疫反应。两种技术均表明对每种菌株的免疫反应在不同时间出现。免疫印迹显示与首次接种的菌株的结合比与第二次接种的菌株更强（P < 0.05）。这些数据证实，子宫感染期间对兽疫链球菌的免疫反应部分具有菌株特异性。
