Ortiz-Plata Alma, Nader-Kawachi Juan, Guevara Jorge, Sandoval Carlos, Rembao Daniel, de la Cruz Hernandez-Hernandez Fidel
Department of Neuropathology, Neurology and Neurosurgery National Institute, Mexico City, Mexico.
Brain Res Mol Brain Res. 2005 Jun 13;137(1-2):55-62. doi: 10.1016/j.molbrainres.2005.02.022. Epub 2005 Apr 7.
Gene expression in frontal, occipital, and hippocampal regions of rat brains at 15 min of ischemic injury was studied in a rat model by producing focal cerebral ischemia through middle cerebral artery (MCA) occlusion without reperfusion. Catalase, epithelial glycoprotein (EGP-314), cytochrome C oxidase-subunit 1, ribosomal L31 protein, and ceruloplasmin were found to be differentially expressed. Specific primers were designed to study this newly reported brain EGP-314, a cellular adhesion molecule involved in cell-cell and cell-extracellular matrix interactions and related with cytoskeletal organization, differentiation, and proliferation. In the frontal and occipital lobes, EGP-314 expression was low in control and ischemic conditions and increased in sham injured conditions, whereas in the hippocampal region its expression was induced only by ischemia. In situ hybridization and immunohistochemistry revealed that EGP-314 mRNA and the protein were present in the ischemic hippocampus pyramidal neurons. DNA fragmentation was demonstrated by TUNEL and LM-PCR analysis in hippocampus region. TUNEL positive pyramidal neurons were observed at 15 min of ischemia. DNA ladder was found at 12 and 15 min of ischemia.
在大鼠模型中，通过大脑中动脉（MCA）闭塞造成局灶性脑缺血且不进行再灌注，研究缺血损伤15分钟时大鼠脑额叶、枕叶和海马区的基因表达。发现过氧化氢酶、上皮糖蛋白（EGP - 314）、细胞色素C氧化酶亚基1、核糖体L31蛋白和铜蓝蛋白存在差异表达。设计了特异性引物来研究这种新报道的脑EGP - 314，它是一种参与细胞间和细胞与细胞外基质相互作用并与细胞骨架组织、分化和增殖相关的细胞粘附分子。在额叶和枕叶，EGP - 314在对照和缺血条件下表达较低，在假损伤条件下表达增加，而在海马区其表达仅由缺血诱导。原位杂交和免疫组织化学显示，EGP - 314 mRNA和蛋白存在于缺血海马锥体神经元中。通过TUNEL和LM - PCR分析在海马区证实了DNA片段化。在缺血15分钟时观察到TUNEL阳性锥体神经元。在缺血12分钟和15分钟时发现了DNA梯带。
