Dalvi R R
Toxicology Laboratory, School of Veterinary Medicine, Tuskegee University, AL 36088.
Toxicol Lett. 1992 May;60(3):299-305. doi: 10.1016/0378-4274(92)90288-u.
Studies were conducted to examine the effect of a single and repeated administrations of garlic oil (diallyl sulfide) on Phase I and Phase II biotransformation enzymes in rats. Adult, male Sprague-Dawley rats treated with a single dose of garlic oil (500 mg/kg i.p.) showed a significant depression of hepatic cytochrome P-450, aminopyrine N-demethylase and aniline hydroxylase while microsomal protein content, cytochrome b5, NADPH-cytochrome c reductase, benzphetamine N-demethylase and cytosolic glutathione, S-transferase remained unaffected 24 h following the treatment. Although certain microsomal enzymes were depressed, there was no liver damage caused by garlic oil as judged by the putative serum enzyme test. On the other hand, daily administration of garlic oil (50 mg/kg i.p. for 5 days) produced a significant increase in hepatic cytochrome P-450, aminopyrine N-demethylase and benzphetamine N-demethylase activities, but not in the rest of the aforementioned parameters of biotransformation reactions. These data indicate that the effect of garlic oil on the hepatic drug-metabolizing enzyme system is dose-dependent.
开展了多项研究，以考察单次及重复给予大蒜油（二烯丙基硫醚）对大鼠Ⅰ相和Ⅱ相生物转化酶的影响。成年雄性斯普拉格-道利大鼠经单次给予大蒜油（500毫克/千克，腹腔注射）后，肝微粒体细胞色素P-450、氨基比林N-脱甲基酶和苯胺羟化酶显著降低，而微粒体蛋白含量、细胞色素b5、NADPH-细胞色素c还原酶、苄非他明N-脱甲基酶和胞质谷胱甘肽S-转移酶在给药后24小时未受影响。尽管某些微粒体酶降低，但通过假定的血清酶检测判断，大蒜油未造成肝损伤。另一方面，每日给予大蒜油（50毫克/千克，腹腔注射，共5天）使肝细胞色素P-450、氨基比林N-脱甲基酶和苄非他明N-脱甲基酶活性显著增加，但上述生物转化反应的其他参数未增加。这些数据表明，大蒜油对肝药物代谢酶系统的影响呈剂量依赖性。
