Edwards Howell G M, Munshi Tasnim
Chemical and Forensic Sciences, School of Pharmacy, University of Bradford, Bradford BD7 1DP, West Yorkshire, UK.
Anal Bioanal Chem. 2005 Jul;382(6):1398-406. doi: 10.1007/s00216-005-3271-8. Epub 2005 Jun 11.
This paper reviews the contributions of analytical Raman spectroscopy to the non-destructive characterisation of biological materials of relevance to forensic science investigations, including the sourcing of resins and the identification of the biodegradation of art and archaeological artefacts. The advantages of Raman spectroscopy for non-destructive analysis are well-appreciated; however, the ability to record molecular information about organic and inorganic species present in a heterogeneous specimen at the same time, the insensitivity of the Raman scattering process to water and hydroxyl groups, which removes the necessity for sample desiccation, and the ease of illumination for samples of very small and very large sizes and unusual shapes are also apparent. Several examples are used to illustrate the application of Raman spectroscopic techniques to the characterisation of forensic biomaterials and for the preservation of cultural heritage through case studies in the following areas: wall-paintings and rock art, human and animal tissues and skeletal remains, fabrics, resins and ivories.
本文综述了分析拉曼光谱在与法医学调查相关的生物材料无损表征方面的贡献，包括树脂的来源追踪以及艺术品和考古文物生物降解的鉴定。拉曼光谱用于无损分析的优势已得到广泛认可；然而，它同时记录异质样本中有机和无机物种分子信息的能力、拉曼散射过程对水和羟基不敏感（从而无需对样品进行干燥处理）以及对非常小和非常大尺寸及形状异常的样品进行照明的便捷性也很明显。通过以下领域的案例研究，使用了几个例子来说明拉曼光谱技术在法医生物材料表征以及文化遗产保护方面的应用：壁画和岩画、人类和动物组织及骨骼遗骸、织物、树脂和象牙。
