[地塞米松对体外培养的小鼠腭突内侧边缘上皮细胞融合的影响]
[Influence of dexamethasone on fusion of embryonic palatal medial edge epithelium in mouse palatal shelves in vitro].
作者信息
Huang Lei, Shi Bing, Sun Jin-Hu, Wang Yan
机构信息
Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2005 Apr;23(2):103-5.
OBJECTIVE
To study the effect of dexamethasone on the differentiation and proliferation of type A mouse palatal medial edge epithelial cells when there is type A mouse embryonic palatal mesenchymal cells.
METHODS
The mouse palatal shelves were harvested from a female mouse of gestation day 14 by microsurgical dissection and cultured in vitro. The differentiation was investigated through microscope and transmission electron microscope under condition of the palatal shelves fusion.
RESULTS
Dexamethasone promoted the palatal medial edge epithelium differentiated into squamause epithelium and affected normal development and obstructed the fusion of mouse palatal shelves.
CONCLUSION
The results of histological observation indicate that dexamethasone promotes the proliferation of palatal meseuchymal cells and inhibits the normal differentiation of palatal medial edge epithelial cells, which results in cleft palate.
目的
研究在有A型小鼠胚胎腭间充质细胞存在时地塞米松对A型小鼠腭中边缘上皮细胞分化和增殖的影响。
方法
通过显微手术从妊娠第14天的雌性小鼠获取腭板并进行体外培养。在腭板融合条件下通过显微镜和透射电子显微镜研究分化情况。
结果
地塞米松促进腭中边缘上皮分化为鳞状上皮，影响正常发育并阻碍小鼠腭板融合。
结论
组织学观察结果表明，地塞米松促进腭间充质细胞增殖并抑制腭中边缘上皮细胞的正常分化，从而导致腭裂。
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