[来自患龋和无龋个体的变形链球菌分离株乳酸脱氢酶活性的研究]
[Study on lactate dehydrogenase activity of Streptococcus mutans isolates derived from caries-active and caries-free individuals].
作者信息
Yang De-Qin, Liu Tian-Jia, Zhou Xue-Dong, He Kui-Fan, Li Song, Zhuang Heng
机构信息
Key Lab. of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2005 Apr;23(2):116-8.
OBJECTIVE
To preliminarily investigate the relationship between the Lactate dehydrogenase (LDH) activity of Streptococcus mutans (S. mutans) and dental caries initiation.
METHODS
100 S. mutans strains derived from caries-active and caries-free individuals were cultured in BHI medium supplemented with glucose. Cells were extracted and ruptured, and the extracted liquid protein was quantified with Coomassie brilliant blue G250 staining methods. LDH activity was assayed using the pyruvate-dependent oxidation of NADH-with and without FDP.
RESULTS
LDH activity of the two groups strains had no difference (P > 0.05), but the distribution of differ class enzyme activity strains in the two groups was different (P < 0.05).
CONCLUSION
LDH activity of S. mutans is correlated to the initiation of dental caries to some extent. The measurement methods in this study can be applied in preliminary quantitation of LDH activity and the screening of S. mutans strains.
目的
初步探究变形链球菌（S. mutans）的乳酸脱氢酶（LDH）活性与龋齿发生之间的关系。
方法
将来自患龋活跃个体和无龋个体的100株变形链球菌菌株在添加葡萄糖的脑心浸液（BHI）培养基中培养。提取并裂解细胞，用考马斯亮蓝G250染色法对提取的液体蛋白进行定量。使用有无果糖二磷酸（FDP）时NADH的丙酮酸依赖性氧化来测定LDH活性。
结果
两组菌株的LDH活性无差异（P>0.05），但两组中不同类酶活性菌株的分布不同（P<0.05）。
结论
变形链球菌的LDH活性在一定程度上与龋齿的发生相关。本研究中的测量方法可用于LDH活性的初步定量及变形链球菌菌株的筛选。
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