Kamiya R U, Napimoga M H, Rosa R T, Höfling J F, Gonçalves R B
Microbiology and Immunology Laboratory, Dentistry School of Piracicaba, State University of Campinas, Brazil.
Oral Microbiol Immunol. 2005 Feb;20(1):20-4. doi: 10.1111/j.1399-302X.2005.00186.x.
Relationships between genetic diversity and mutacin production in Streptococcus mutans were evaluated in 319 clinical isolates from eight caries-affected and eight caries-free individuals. The isolates were submitted to mutacin typing and AP-PCR (arbitrarily primed polymerase chain reaction) assay. The mutacin production was detected for 12 Streptococcus sp. indicator strains. Results showed significant variations in the mutacin production profiles and the inhibitory spectra of both groups. A possible association was seen between mutacin activity and the distinct patterns of Streptococcus sp. colonization in the two groups. Genotyping by AP-PCR using the primers OPA-02 and OPA-13 revealed 101 distinct genotypes against 48 phenotypes identified by mutacin typing. No correlation was observed between the inhibitory spectra of mutacin and genotypic similarities based on AP-PCR analyses. According to our results, strains of the same S. mutans genotype showed different mutacin profiles, suggesting a high degree of interstrain diversity. In conclusion, mutacin production seems to be of clinical importance in the colonization of S. mutans and is highly diversified in the S. mutans species.
对来自8名患龋个体和8名无龋个体的319株临床分离株进行了变形链球菌遗传多样性与变链菌素产生之间关系的评估。将这些分离株进行变链菌素分型和任意引物聚合酶链反应（AP-PCR）分析。检测了12株链球菌属指示菌株的变链菌素产生情况。结果显示两组的变链菌素产生谱和抑制谱存在显著差异。在两组中，变链菌素活性与链球菌属定殖的不同模式之间可能存在关联。使用引物OPA-02和OPA-13通过AP-PCR进行基因分型，针对通过变链菌素分型鉴定的48种表型揭示了101种不同的基因型。基于AP-PCR分析，未观察到变链菌素抑制谱与基因型相似性之间的相关性。根据我们的结果，相同变形链球菌基因型的菌株表现出不同的变链菌素谱，表明菌株间存在高度多样性。总之，变链菌素产生似乎在变形链球菌定殖中具有临床重要性，并且在变形链球菌种内高度多样化。
