[镉诱导人外周血淋巴细胞中金属硫蛋白异构体基因表达]

[Metallothionein isoforms gene expression induced by cadmium in human peripheral blood lymphocytes].

作者信息

Chang Xiu-li, Jin Tai-yi, Zhou Yuan-fen

机构信息

School of Public Health, Fudan University, Shanghai 200032, China.

出版信息

Wei Sheng Yan Jiu. 2005 Mar;34(2):137-40.

PMID:15952643

Abstract

OBJECTIVE

To explore gene expression of metallothionein 1 (MT-1) isoforms in human peripheral blood lymphocytes.

METHODS

mRNA of expression representing the seven active subtypes of MT-1 gene was determined in cultured human peripheral blood lymphocytes (HPBLs) before and after exposure to cadmium by quantitative RT-PCR.

RESULTS

Basal expression of MT-1X, MT-1A in HPBLs was similar to expression of the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase. In contrast, the basal gene expression of MT-1H, MT-1F, MT-1E, MT-1G was a little transcript in HPBLs. No signal was detected for the MT-1B. There was a sex difference (P < 0.05) in basal gene expression of MT-1E. The level of gene expression of MT-1A, MT-1E, MT-1F, MT-1G, MT-1H and MT-1X was significantly increased (P < 0.05), but not the level of MT-1B after exposure to cadmium.

CONCLUSION

It was suggested that gene expression of MT-1F, MT-1G, MT-1H and 1X in HPBLs could be used as potential exposure biomarker.

摘要

目的

探讨金属硫蛋白1（MT-1）亚型在人外周血淋巴细胞中的基因表达。

方法

通过定量逆转录聚合酶链反应（RT-PCR）测定培养的人外周血淋巴细胞（HPBLs）在镉暴露前后代表MT-1基因七种活性亚型的表达mRNA。

结果

HPBLs中MT-1X、MT-1A的基础表达与管家基因甘油醛-3-磷酸脱氢酶的表达相似。相比之下，MT-1H、MT-1F、MT-1E、MT-1G在HPBLs中的基础基因表达量较少。未检测到MT-1B的信号。MT-1E的基础基因表达存在性别差异（P<0.05）。镉暴露后，MT-1A、MT-1E、MT-1F、MT-1G、MT-1H和MT-1X的基因表达水平显著升高（P<0.05），但MT-1B的水平未升高。