人血小板前体细胞（CD61+巨核细胞）中的金属硫蛋白。

Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes.

作者信息

Rahman M T, De Ley M

机构信息

Department of Chemistry, Laboratory for Biochemistry, Katholieke Universiteit Leuven, Leuven, Heverlee, Belgium.

出版信息

Cell Biol Toxicol. 2008 Jan;24(1):19-25. doi: 10.1007/s10565-007-9012-3. Epub 2007 Apr 28.

DOI:10.1007/s10565-007-9012-3

PMID:17468938

Abstract

The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61(+) cells at 48 h post-treatment with 100 micromol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar cell populations left untreated with zinc.

摘要

对从人脐带血中分离出的血小板前体（巨核细胞）中金属硫蛋白（MT）的体外生物合成进行了研究。通过使用小鼠抗MT单克隆抗体进行免疫组织化学染色，证实了磁性细胞分选分离的CD61(+)巨核细胞中MT的生物合成和诱导。在细胞核和细胞质区域均检测到MT的存在。使用逆转录聚合酶链反应（RT-PCR），在用100微摩尔/升锌补充剂处理48小时后，在CD61(+)细胞中观察到总MT转录本的体外上调/诱导。在未用锌处理的相似细胞群体中检测到七种亚型特异性mRNA，即MT-1A、MT-1B、MT-1E、MT-1G、MT-1H、MT-1X和MT-2A。