氧化葡萄糖酸杆菌产葡聚糖糊精酶的三因素响应面优化

Three-factor response surface optimization of the production of dextran dextrinase by Gluconobacter oxydans.

作者信息

Naessens M, Vercauteren R, Vandamme E J

机构信息

Department of biochemical and microbial technology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.

出版信息

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2001;66(3a):303-6.

PMID:15954607

Abstract

Response surface methodology (RSM) and a five-level three-factor central composite rotatable design (CCRD) were used to evaluate the effect of glycerol and peptone concentration and initial pH on dextran dextrinase (DDase) production by Gluconobacter oxydans. Optimal fermentation conditions were 20.59 g/l of glycerol, 6.67 g/l of mycological peptone and an initial pH of 6.14. The predicted DDase yield of the optimised fermentation was 0.207 U/ml, whereas an actual experimental yield of 0.208 +/- 0.025 U/ml was obtained.

摘要

采用响应面法（RSM）和五水平三因素中心复合旋转设计（CCRD），评估甘油和蛋白胨浓度以及初始pH值对氧化葡萄糖酸杆菌产葡聚糖糊精酶（DDase）的影响。最佳发酵条件为甘油20.59 g/l、真菌蛋白胨6.67 g/l和初始pH值6.14。优化发酵的预测DDase产量为0.207 U/ml，而实际实验产量为0.208±0.025 U/ml。