侵袭内阿米巴：使用血清替代品进行体外纯培养包囊化

Entamoeba invadens: in vitro axenic encystation with a serum substitute.

作者信息

Barrón-González M P, Villarreal-Treviño L, Verduzco-Martínez J A, Mata-Cárdenas B D, Morales-Vallarta M R

机构信息

Departamento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Mexico.

出版信息

Exp Parasitol. 2005 Jul;110(3):318-21. doi: 10.1016/j.exppara.2005.03.021.

DOI:10.1016/j.exppara.2005.03.021

PMID:15955331

Abstract

The current media for axenic cultivation of Entamoeba histolytica and Entamoeba invadens are supplemented with bovine or equine serum, which provides several essential nutrients to amoebas. Serum has also been considered an essential component in encystation media for E. invadens. A substitute of serum, PACSR has been described as an alternative for growth of E. histolytica and also maintains growth of E. invadens. When PACSR was used instead of serum for encystation of E. invadens the efficiency was the same as for serum. Our present data show that PACSR can support the growth and induction of encystation of E. invadens strain IP-1.

摘要

目前用于溶组织内阿米巴和侵袭内阿米巴无菌培养的培养基中添加了牛血清或马血清，这些血清为阿米巴提供了几种必需营养物质。血清也被认为是侵袭内阿米巴包囊化培养基中的一种必需成分。一种血清替代品——富含脯氨酸的酸性肽（PACSR）已被描述为溶组织内阿米巴生长的替代物，并且能维持侵袭内阿米巴的生长。当使用PACSR代替血清进行侵袭内阿米巴的包囊化时，其效率与使用血清时相同。我们目前的数据表明，PACSR能够支持侵袭内阿米巴IP - 1株的生长和包囊化诱导。