Uribe-Lorío Lorena, Mora Marielos, Stock S Patricia
Centro de Biología Celular y Molecular, Universidad de Costa Rica, Costa Rica.
J Invertebr Pathol. 2005 Mar;88(3):226-31. doi: 10.1016/j.jip.2005.01.007. Epub 2005 Feb 19.
A survey of entomopathogenic nematodes was conducted in the north Pacific (Guanacaste Conservation Area) and southeast Caribbean (Gandoca-Manzanillo Natural Refuge) regions of Costa Rica. Out of a total of 41 soil samples, 5 were positive for entomopathogenic nematodes (20.5%), with 3 (12.3%) containing Steinernema and 2 (8.2%) Heterorhabditis isolates. Morphological and molecular studies were undertaken to characterize these isolates. The Heterorhabditis isolates were identified as Heterorhabditis indica and the three Steinernema isolates were identified as two new undescribed species. H. indica was recovered from a coastal dry forest. Steinernema n. sp. 1 was isolated from a rainforest valley, between volcanoes. Steinernema sp. n. 2 was isolated from sand dunes in the Caribbean Coast (Punta Uva) near the rainforest strip along the coast. Although limited to two geographic regions, this study suggests entomopathogenic nematodes may be diverse and perhaps widely distributed in Costa Rica. A more intensive survey, covering all geographic regions is currently undergoing.
在哥斯达黎加的北太平洋地区（瓜纳卡斯特自然保护区）和东南加勒比地区（甘多卡-曼萨尼约自然保护区）开展了一次昆虫病原线虫调查。在总共41份土壤样本中，5份对昆虫病原线虫呈阳性（20.5%），其中3份（12.3%）含有斯氏线虫属，2份（8.2%）含有异小杆线虫属分离物。开展了形态学和分子研究以对这些分离物进行特征描述。异小杆线虫属分离物被鉴定为印异小杆线虫，3份斯氏线虫属分离物被鉴定为2个未描述的新物种。印异小杆线虫是从沿海干旱森林中分离得到的。新种斯氏线虫1号是从两座火山之间的雨林山谷中分离得到的。新种斯氏线虫2号是从加勒比海岸（蓬塔乌瓦）靠近沿海雨林带的沙丘中分离得到的。尽管该研究仅限于两个地理区域，但表明昆虫病原线虫在哥斯达黎加可能具有多样性且分布广泛。目前正在开展一项覆盖所有地理区域的更深入调查。
