Trammell Scott A, Spano Anthony, Price Ronald, Lebedev Nikolai
Center for Bio/Molecular Science & Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, DC 20375, USA.
Biosens Bioelectron. 2006 Jan 15;21(7):1023-8. doi: 10.1016/j.bios.2005.03.015. Epub 2005 Jun 13.
A new type of monolayer of photosynthetic reaction centers (RC) with the primary donor facing the carbon electrode has been constructed using a new bifunctional linker and genetically engineered protein. Comparison of protein in two different orientations with linkers binding to the opposite sides of the protein demonstrates the possibility of utilizing the constructed surfaces as photoelectronic devices. The results show improvement of the electron transfer efficiency when RC is bound with the primary donor (P) facing the electrode (P-side). In either protein orientation, electron transfer within the protein is unidirectional and when applying a voltage RC operates as a photorectifier. Electron transfer between the protein and carbon electrodes in the constructed devices is most likely occurring by tunneling.
利用一种新型双功能连接体和基因工程蛋白构建了一种新型的光合反应中心（RC）单层，其初级供体面向碳电极。将两种不同取向的蛋白质与连接在蛋白质相对两侧的连接体进行比较，证明了将构建的表面用作光电器件的可能性。结果表明，当RC以初级供体（P）面向电极（P侧）结合时，电子转移效率得到提高。在任何一种蛋白质取向中，蛋白质内部的电子转移都是单向的，并且在施加电压时，RC作为光整流器工作。构建的器件中蛋白质与碳电极之间的电子转移很可能是通过隧道效应发生的。
