Tondreau Tatiana, Meuleman Nathalie, Delforge Alain, Dejeneffe Marielle, Leroy Rita, Massy Martine, Mortier Christine, Bron Dominique, Lagneaux Laurence
Jules Bordet Institute, 121 Bd de Waterloo, 1000 Brussels, Belgium.
Stem Cells. 2005 Sep;23(8):1105-12. doi: 10.1634/stemcells.2004-0330. Epub 2005 Jun 13.
In this study, we used a common procedure to assess the potential of mobilized peripheral blood (MPB) and umbilical cord blood (UCB) as sources of mesenchymal stem cells (MSCs) in comparison with bone marrow (BM). We tested three methods: plastic adhesion supplemented with 5% of BM-MSC conditioned medium, unsupplemented plastic adhesion, and selection of CD133-positive cells. MSCs derived from MPB or UCB are identified by their positive expression of mesenchymal (SH2, SH3) and negative expression of hematopoietic markers (CD14, CD34, CD45, HLA-DR). We observed that the CD133-positive cell fraction contains more MSCs with high proliferative potential. Placed in appropriate conditions, these cells proved their capacity to differentiate into adipocytes, osteocytes, chondrocytes, and neuronal/glial cells. MPB- and UCB-MSCs express Oct4, a transcriptional binding factor present in undifferentiated cells with high proliferative capacity. The selection of CD133-positive cells enabled us to obtain a homogeneous population of MSCs from UCB and MPB. These sources may have a major clinical importance thanks to their easy accessibility.
在本研究中，我们采用一种常规方法，将动员外周血（MPB）和脐带血（UCB）作为间充质干细胞（MSC）来源的潜力与骨髓（BM）进行比较。我们测试了三种方法：补充5%骨髓间充质干细胞条件培养基的塑料贴壁法、未补充条件培养基的塑料贴壁法以及CD133阳性细胞分选法。源自动员外周血或脐带血的间充质干细胞通过其间充质标志物（SH2、SH3）阳性表达和造血标志物（CD14、CD34、CD45、HLA-DR）阴性表达来鉴定。我们观察到，CD133阳性细胞组分含有更多具有高增殖潜力的间充质干细胞。置于适当条件下，这些细胞证明了它们分化为脂肪细胞、骨细胞、软骨细胞和神经/胶质细胞的能力。动员外周血和脐带血间充质干细胞表达Oct4，Oct4是一种存在于具有高增殖能力的未分化细胞中的转录结合因子。CD133阳性细胞分选法使我们能够从脐带血和动员外周血中获得均一的间充质干细胞群体。由于其易于获取，这些来源可能具有重要的临床意义。
