Yue Feng, Ma Wen-li, Song Yan-bin, Shi Rong, Zhang Bao, Zheng Wen-ling
Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Jun;25(6):647-50.
To study the inhibitory effect of small interfering RNA (siRNA) targeting c-myc gene in K562 cells.
siRNAs targeting the site 1357 of c-myc mRNA was designed and synthesized. In vitro cultured K562 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT)-PCR, cell count, MTT assay and fluorescence-activated cell sorting.
Compared with the negative and blank control group, the transfection group showed marked decrease in the c-myc expression and the K562 cells exhibited increased apoptosis rate.
RNA interference can effectively inhibit c-myc expression and induce apoptosis in K562 cells.
研究靶向c-myc基因的小干扰RNA（siRNA）对K562细胞的抑制作用。
设计并合成靶向c-myc mRNA第1357位点的siRNAs。用脂质体2000转染体外培养的K562细胞，通过逆转录聚合酶链反应（RT-PCR）、细胞计数、MTT法和荧光激活细胞分选检测抑制效果。
与阴性对照组和空白对照组相比，转染组c-myc表达明显降低，K562细胞凋亡率升高。
RNA干扰可有效抑制K562细胞中c-myc的表达并诱导其凋亡。
