Weisel Katja C, Moore Malcolm A S
University of Tübingen, Department of Hematology, Oncology, and Immunology, Otfried-Müller-Strasse 10, 72076 Tübingen, Germany.
Ann N Y Acad Sci. 2005 Jun;1044:51-9. doi: 10.1196/annals.1349.007.
The hematopoietic system interacts with a supportive stromal environment allowing maintenance and differentiation of hematopoietic stem cells (HSCs). The aorta-gonado-mesonephros (AGM) region serves as a unique embryonic microenvironment, generating the first adult repopulating HSCs in the mouse embryo. To eludicate factors involved in hematopoietic support and induction of hematopoietic differentiation, we isolated more than 100 stromal cell clones derived from the AGM region of embryonic day (E) 10.5 mouse embryos for functional and genetic analysis. Selected isolated AGM stromal cell lines are highly efficient in supporting maintenance and expansion of mouse and human hematopoietic stem and progenitor cells. In addition, we can demonstrate for the first time that AGM stromal cell lines are also potent inducers of hematopoietic differentiation of murine embryonic stem cells. Stromal gene array analysis has identified genes that could play a role in hematopoietic support.
造血系统与支持性的基质环境相互作用，以维持造血干细胞（HSCs）并使其分化。主动脉-性腺-中肾（AGM）区域是一个独特的胚胎微环境，在小鼠胚胎中产生第一批能够重建造血的成年HSCs。为了阐明参与造血支持和诱导造血分化的因素，我们从胚胎第10.5天（E10.5）小鼠胚胎的AGM区域分离了100多个基质细胞克隆，用于功能和基因分析。所选的分离AGM基质细胞系在支持小鼠和人类造血干细胞及祖细胞的维持和扩增方面效率很高。此外，我们首次证明AGM基质细胞系也是小鼠胚胎干细胞造血分化的有效诱导剂。基质基因阵列分析已鉴定出可能在造血支持中发挥作用的基因。
