[青少年特发性脊柱侧凸患者顶点关节突中I型胶原蛋白的表达]
[Expression of collagen type I in the articular processes of apex from adolescent idiopathic scoliosis patients].
作者信息
Qian Wen-wei, Qiu Gui-xing, Wu Zhi-hong, Liu Yong, Wang Yi-peng, Zhang Jian-guo, Weng Xi-sheng, Yang Xin-yu
机构信息
Department of Orthopaedics Surgery, PUMC Hospital, CAMS and PUMC, Beijing, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Apr;27(2):138-42.
OBJECTIVE
To investigate the characteristic of the expression of collagen type I in the articular processes of apex from adolescent idiopathic scoliosis (AIS) patients.
METHODS
The apical processes of 11 AIS patients were collected. The techniques of HE staining and In-cell Western were adopted in this research. We studied the pathological changes of the apical processes and collected and cultured the osteoblast from the apical processes on both sides in vitro separately. After cell identification, we compared the secretion of collagen type I in the osteoblast from concave side to that of convex side.
RESULTS
The cartilages of the apical processes showed some signs of regression. The secretion of collagen type I of the osteoblast in vitro from the concave side of the apical articular process was significantly higher than that from the convex side (P < 0.05).
CONCLUSION
Significant difference of the gene expression of the osteoblasts from the apical process exists between the concave side and the convex side.
目的
探讨青少年特发性脊柱侧凸（AIS）患者顶椎关节突中Ⅰ型胶原蛋白的表达特征。
方法
收集11例AIS患者的顶椎关节突。本研究采用苏木精-伊红（HE）染色和细胞内蛋白质免疫印迹技术。我们研究了顶椎关节突的病理变化，并分别在体外从两侧顶椎关节突收集并培养成骨细胞。细胞鉴定后，比较顶椎关节突凹侧与凸侧成骨细胞中Ⅰ型胶原蛋白的分泌情况。
结果
顶椎关节突软骨出现一些退变迹象。顶椎关节突凹侧体外培养的成骨细胞中Ⅰ型胶原蛋白的分泌明显高于凸侧（P < 0.05）。
结论
顶椎关节突凹侧与凸侧成骨细胞的基因表达存在显著差异。
Zotero 插件