Qiu Gui-xing, Li Qi-yi, Liu Yong, Wu Zhi-hong, Zhang Jian-guo, Wang Yi-peng, Weng Xi-sheng, Shen Jian-xiong, Wang Ting
Department of Orthopeadics, Peking Union Medical College Hospital, Beijing 100730, China.
Zhonghua Wai Ke Za Zhi. 2006 Oct 15;44(20):1422-6.
To look into the character of the expression of collagen type II and transforming growth factor beta1 (TGF-beta1), basic fibroblast growth factor (bFGF) in the apical articular process cartilages of adolescent idiopathic scoliosis (AIS) and congenital scoliosis (CS) patients.
The articular processes of 22 AIS and 18 CS were collected. The techniques of HE staining, immunohistochemistry and in situ hybridization were adopted in this research. By comparing the apical processes with the end processes, the convex processes with the concave processes, the AIS processes with CS processes, the pathological changes of the articular process cartilages of these patients and the distribution of collagen type II and TGF-beta1, bFGF in them were studied. The images of immunohistochemistry and in situ hybridization were input into the image analysis system and were analyzed semi-quantitatively. The SAS software (8.01) was adopted, and P < 0.05 was defined as the significant level.
The expression of collagen type II and TGF-beta1, bFGF in AIS was similar to CS: the concave sides of apexes were higher than the convex sides. The comparisons had statistical significance. There was no statistical significance between upper and lower end vertebrae in convex and concave sides, between convex and concave sides in upper and lower end vertebrae. The apical vertebrae were significantly higher than the ipsilateral sides of upper or lower end vertebrae for collagen type II. There was no statistical difference of the expression at the concave, convex, upper, lower end vertebrae between AIS and CS.
The cartilages of the apical processes show some signs of regression and hypoplasia in scoliosis. The concave side is more severe than the convex side. Increase of collagen type II and TGF-beta1, bFGF in the concave sides of apical processes in scoliosis may be the results of reconstruction of extracellular matrix and the compensation reactions which are caused by abnormal biomechanical forces such as compressive stresses. Compressive stress on the concave sides has more influences on the expression of collagen type II than tensile stress on the convex sides.
探讨青少年特发性脊柱侧凸（AIS）和先天性脊柱侧凸（CS）患者顶椎关节突软骨中Ⅱ型胶原蛋白、转化生长因子β1（TGF-β1）和碱性成纤维细胞生长因子（bFGF）的表达特征。
收集22例AIS和18例CS患者的关节突。本研究采用苏木精-伊红（HE）染色、免疫组织化学和原位杂交技术。通过比较顶椎与终椎、凸侧与凹侧、AIS患者关节突与CS患者关节突，研究这些患者关节突软骨的病理变化以及Ⅱ型胶原蛋白、TGF-β1和bFGF在其中的分布。将免疫组织化学和原位杂交图像输入图像分析系统进行半定量分析。采用SAS软件（8.01），以P<0.05为差异有统计学意义。
AIS患者中Ⅱ型胶原蛋白、TGF-β1和bFGF的表达与CS患者相似：顶椎凹侧高于凸侧。比较具有统计学意义。凸侧和凹侧的上端椎和下端椎之间、上端椎和下端椎的凸侧与凹侧之间无统计学意义。Ⅱ型胶原蛋白在顶椎显著高于上端椎或下端椎的同侧。AIS和CS患者在凹侧、凸侧、上端椎、下端椎的表达无统计学差异。
脊柱侧凸患者顶椎关节突软骨出现一些退变和发育不全的迹象。凹侧比凸侧更严重。脊柱侧凸顶椎凹侧Ⅱ型胶原蛋白、TGF-β1和bFGF的增加可能是细胞外基质重建以及由压缩应力等异常生物力学力引起的代偿反应的结果。凹侧的压缩应力比凸侧的拉伸应力对Ⅱ型胶原蛋白表达的影响更大。
