Feng Wenling, Zheng Wang
Department of Orthopedic Surgery, Third Hospital of Hebei Medical University, Shijiazhuang Hebei, 050051, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 May;19(5):350-3.
To validate the advantage of repairing bone defect by staphylococcus aureus injection carried in collagen membrane.
Twenty-four adult New Zealand rabbits were divided into two groups randomly. After the experimental model of standard bone defect had been made by operation, collagen membrane/staphylococcus aureus injection and staphylococcus aureus injection with the same quantity were transplanted in bone defect areas of the two groups respectively. The reconstructed tissues were observed by general method, X-ray, histology, and immunohistochemistry at 2nd, 4th, 6th, 8th week respectively.
The experimental group showed that new bone proliferated distinctly in bone defect area and the proliferation lasted long, and no excessive connective tissue in defect area. X-ray observation showed that there was continual callus growth in transplantation area in early stage and the distribution of new bones was even in the group. Histological observation showed that there were many new bone growth centers in bone defect area, trabecular bones were sequentially distributed, and mature bone replacement was complete. Immunohistochemical examination showed that bone morphogenetic protein (BMP) could be seen for a long time and BMP took up a large part in the new bone tissues.
Collagen membrane could prevent parenchyma from penetrating into bone defect area and provide room for new bone growth. As the carrier of staphylococcus, collagen membrane could reduce the overflow of staphylococcus and improve its curative effect as well.
验证胶原膜携带金黄色葡萄球菌注射修复骨缺损的优势。
将24只成年新西兰兔随机分为两组。手术制作标准骨缺损实验模型后，分别将胶原膜/金黄色葡萄球菌注射物和等量的金黄色葡萄球菌注射物植入两组的骨缺损区。分别在第2、4、6、8周采用大体观察、X线、组织学及免疫组织化学方法观察重建组织。
实验组骨缺损区新骨明显增生且增生持续时间长，缺损区无过多结缔组织。X线观察显示早期移植区有连续骨痂生长，且新骨分布均匀。组织学观察显示骨缺损区有多个新骨生长中心，小梁骨依次分布，成熟骨替代完全。免疫组织化学检查显示骨形态发生蛋白（BMP）可长时间存在，且在新骨组织中占很大比例。
胶原膜可阻止实质组织长入骨缺损区，为新骨生长提供空间。作为金黄色葡萄球菌的载体，胶原膜可减少金黄色葡萄球菌的溢出，提高其治疗效果。
