Zhuo Benhui, Jiang Hebi, Qu Ping
Children's Hospital, Chongqing University of Medical Sciences, Chongqing, 400014, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 May;19(5):373-6.
To investigate the neural markers' expression in the differentiation of marrow stromal stem cells (MSCs) into neural cells.
Rats MSCs were expanded as undifferentiated cells in vitro for 5 to 7 generations and cultured in a modified neuronal medium (MNM) after 24 hours of all-trans retinoid acid (ATRA) pretreatment. Immunocytochemistry was used to detect the expression of nestin, neuron-specific nuclear protein (NeuN), microtubule-associated protein 2 (MAP-2) and glial fibrillary acidic protein (GFAP) at different time points.
After ATRA and MNM treatment, MSCs progressively assumed neuronal morphological characteristics. Nestin occurred first after 24 hours of ATRA treatment; then NeuN expressed after 2 hours of MNM treatment; the last one was MAP-2 and it was detected after 9 hours of MNM treatment. Other markers continuously expressed except that the expression of nestin peaked after 18 hours of MNM induction and remarkably decreased after 36 hours.
ATRA and MNM could promote the differentiation of MSCs into neural cells and the expression of neural-specific markers was consistent with current knowledge regarding the time points of markers expression in the neuronal development which provides a good model in vitro for neuronal development research.
研究骨髓间充质干细胞（MSCs）向神经细胞分化过程中神经标志物的表达情况。
将大鼠MSCs作为未分化细胞在体外扩增5至7代，经全反式维甲酸（ATRA）预处理24小时后，在改良神经元培养基（MNM）中培养。采用免疫细胞化学法检测不同时间点巢蛋白、神经元特异性核蛋白（NeuN）、微管相关蛋白2（MAP-2）和胶质纤维酸性蛋白（GFAP）的表达。
经ATRA和MNM处理后，MSCs逐渐呈现神经元形态特征。ATRA处理24小时后首先出现巢蛋白表达；MNM处理2小时后NeuN表达；最后是MAP-2，在MNM处理9小时后检测到。除巢蛋白在MNM诱导18小时后表达达到峰值，36小时后显著下降外，其他标志物持续表达。
ATRA和MNM可促进MSCs向神经细胞分化，神经特异性标志物的表达与当前关于神经元发育中标志物表达时间点的认识一致，为神经元发育研究提供了良好的体外模型。
