Shang Panfeng, Zhao Yanzong, Yue Zhongjin
Department of Urology, The Second Hospital of Lanzhou University, Lanzhou Gansu, 730030, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 May;19(5):386-8.
To improve arterial anastomosis method for rat renal transplantation.
Renal transplantations were performed on 72 wistar rats. The donor superior mesenteric artery was end-to-end anastomosed to the recipient left renal artery by using of sleeve anastomosis technique. The external diameters of the vessels anastomosed were 0.60 +/- 0.05 mm (left renal artery) or 0.80 +/- 0.07 mm (superior mesenteric artery). The procedure consisted of a guiding suture and two fixing sutures. The guiding suture was used to "telescope" the recipient left renal artery into the donor superior mesenteric artery about 2 millimetre. Two fixing sutures were applied 180 degrees apart from each other and tied. Three sutures passed through all layers of the donor superior mesenteric artery and constricted the vessel lumen, but only penetrated the adventitia of the recipient left renal artery.
The time for arterial anastomoses was approximately 6 to 8 minutes. The renal grafts perfused very well after the recipient left renal artery clamp was removed. Complications included anastomotic hemorrhage (1 case) and thrombosis (1 case). Histologic examination of 34 grafts at different postoperative time ranging from 6 to 30 days revealed that renal artery was fully patent, with no evidence of ischemic injury.
The modified arterial sleeve anastomosis technique is simple and feasible regardless of experimental condition and can be easily performed.
改进大鼠肾移植的动脉吻合方法。
对72只Wistar大鼠进行肾移植。采用套管吻合技术将供体肠系膜上动脉与受体左肾动脉进行端端吻合。吻合血管的外径为0.60±0.05毫米（左肾动脉）或0.80±0.07毫米（肠系膜上动脉）。该操作包括一根引导缝线和两根固定缝线。引导缝线用于将受体左肾动脉“套入”供体肠系膜上动脉约2毫米。两根固定缝线彼此相隔180度并打结。三根缝线穿过供体肠系膜上动脉的所有层并使血管腔收缩，但仅穿透受体左肾动脉的外膜。
动脉吻合时间约为6至8分钟。移除受体左肾动脉夹后，肾移植器官灌注良好。并发症包括吻合口出血（1例）和血栓形成（1例）。对术后6至30天不同时间的34个移植器官进行组织学检查，结果显示肾动脉完全通畅，无缺血性损伤迹象。
改良的动脉套管吻合技术无论实验条件如何都简单可行，且易于操作。
