Hoe C H, Yasin R M, Koh Y T, Thong K L
Microbiology Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
J Appl Microbiol. 2005;99(1):133-40. doi: 10.1111/j.1365-2672.2005.02581.x.
Antimicrobial resistance of Shigella sonnei from Malaysia was determined and subtyping was carried by pulsed-field gel electrophoresis (PFGE) to assess the extent of genetic diversity of these strains.
A total of 62 isolates of S. sonnei from sporadic cases of shigellosis in different parts of Malaysia were studied by antimicrobial susceptibility test and PFGE. Approximately 35.5% of the strains showed resistance to three or more antimicrobial agents. Eight resistant phenotypes, i.e. RI to RVIII, was defined. Resistant phenotype RV and RVIII only appeared in year 2000. PFGE analysis with NotI and XbaI restriction showed that a great heterogeneity existed at the DNA level among Malaysian S. sonnei isolates. Fifty-eight NotI and 61 XbaI-PFGE profiles were observed in 63 S. sonnei isolates, including ATCC 11060 isolate. Drug sensitive isolates displayed very different profiles from drug-resistant isolates, with a few exceptions. Isolates of resistant phenotype RVI (SXTr.TETr.STRr) showed a greater similarity among each other compared with isolates of resistant phenotype RI and drug-sensitive isolates.
Multi-drug-resistant S. sonnei were circulated in different parts of Malaysia and the emergence of new resistant phenotype was observed. Wide genetic variations among Malaysian S. sonnei were observed and the drug-sensitive strains could be differentiated from drug-resistant strains by PFGE.
The present study verifies the usefulness of PFGE in characterizing and comparing strains of S. sonnei. Minor variations among S. sonnei isolates could be detected by PFGE.
测定马来西亚宋内志贺菌的耐药性，并通过脉冲场凝胶电泳（PFGE）进行亚型分析，以评估这些菌株的遗传多样性程度。
对从马来西亚不同地区志贺菌病散发病例中分离出的62株宋内志贺菌进行了药敏试验和PFGE研究。约35.5%的菌株对三种或更多抗菌药物耐药。定义了8种耐药表型，即RI至RVIII。耐药表型RV和RVIII仅在2000年出现。用NotI和XbaI酶切进行PFGE分析表明，马来西亚宋内志贺菌分离株在DNA水平上存在很大的异质性。在63株宋内志贺菌分离株（包括ATCC 11060分离株）中观察到58种NotI和61种XbaI-PFGE图谱。除少数例外，药敏分离株与耐药分离株的图谱差异很大。耐药表型RVI（SXTr.TETr.STRr）的分离株彼此之间的相似性高于耐药表型RI的分离株和药敏分离株。
多重耐药的宋内志贺菌在马来西亚不同地区传播，且观察到新的耐药表型出现。观察到马来西亚宋内志贺菌存在广泛的遗传变异，通过PFGE可将药敏菌株与耐药菌株区分开来。
本研究验证了PFGE在鉴定和比较宋内志贺菌菌株方面的实用性。通过PFGE可检测到宋内志贺菌分离株之间的微小差异。
