Kontro M, Lignell U, Hirvonen M-R, Nevalainen A
Laboratory of Environmental Microbiology, Division of Environmental Health, National Public Health Institute, Kuopio, Finland.
Lett Appl Microbiol. 2005;41(1):32-8. doi: 10.1111/j.1472-765X.2005.01727.x.
Streptomycetes are regarded to prefer neutral to alkaline environmental pH, although they commonly occur at remarkably variable pH and nutritional conditions. Therefore, the dependence of 10 Streptomyces spp. pH tolerance on nutrients was determined.
Ten environmental Streptomyces spp. were grown and sporulated between pH 4.0 and 11.5, at the interval of 1.5, on starch-casein-KNO(3), tryptone-yeast extract-glucose, glycerol-arginine and tryptone-soy agars, and three their modifications. On media with starch and casein; glucose, tryptone and yeast extract; tryptone and soy peptone; and glycerol-arginine and yeast extract strains grew over a broad pH range between 4.0-5.5 and 10.0-11.5. On glycerol-arginine and on medium with Na-propionate, NH(4)NO(3) and yeast extract, streptomycetes grew optimally at pH 7.0 and above. The high organic load enabled the growth over a wide pH range. The sporulation pH ranges followed those for growth.
The high organic load enabled the growth over a wide pH range. The strain-specific differences in sporulation were greater than those caused by pH. The best medium for sporulation contained glucose and tryptone with minerals of glycerol-arginine agar at pH 5.5.
The growth pH ranges, pH ranges for the optimal growth, and sporulation were strongly dependent on nutrients.
链霉菌通常被认为偏好中性至碱性的环境pH值，尽管它们在显著不同的pH值和营养条件下普遍存在。因此，测定了10种链霉菌对pH的耐受性对营养物质的依赖性。
在淀粉 - 酪蛋白 - KNO₃、胰蛋白胨 - 酵母提取物 - 葡萄糖、甘油 - 精氨酸和胰蛋白胨 - 大豆琼脂及其三种改良培养基上，使10种环境链霉菌在pH 4.0至11.5之间以1.5的间隔生长并形成孢子。在含有淀粉和酪蛋白、葡萄糖、胰蛋白胨和酵母提取物、胰蛋白胨和大豆蛋白胨以及甘油 - 精氨酸和酵母提取物的培养基上，菌株在4.0 - 5.5和10.0 - 11.5的较宽pH范围内生长。在甘油 - 精氨酸以及含有丙酸钠、NH₄NO₃和酵母提取物的培养基上，链霉菌在pH 7.0及以上时生长最佳。高有机负荷使得能够在较宽的pH范围内生长。孢子形成的pH范围与生长的pH范围一致。
高有机负荷使得能够在较宽的pH范围内生长。孢子形成的菌株特异性差异大于pH引起的差异。用于孢子形成的最佳培养基是在pH 5.5时含有葡萄糖和胰蛋白胨以及甘油 - 精氨酸琼脂矿物质的培养基。
生长的pH范围、最佳生长的pH范围以及孢子形成强烈依赖于营养物质。
