利用木霉菌株中的一种新的葡糖醛酸聚糖裂解酶活性生产葡糖醛酸寡糖。

Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp. strain.

作者信息

Delattre C, Michaud P, Lion J M, Courtois B, Courtois J

机构信息

Laboratoire des Glucides-EPMV CNRS FRE 2779, IUT/GB, UPJV, Avenue des Facultés, Le Bailly, 80025 Amiens Cedex, France.

出版信息

J Biotechnol. 2005 Sep 10;118(4):448-57. doi: 10.1016/j.jbiotec.2005.04.014.

DOI:10.1016/j.jbiotec.2005.04.014

PMID:15961175

Abstract

Sinorhizobium meliloti M5N1CS synthesizes a homopolymer of glucuronic acids beta-(1,4) linked and variably C2 and/or C3O-acetylated. To obtain beta-Delta-(4,5)-unsaturated oligoglucuronans, various acetylated forms of this bacterial polymer were cleaved by a Trichoderma sp. GL2 glucuronan lyase. Oligomers with polymerization degrees up to 8 were then produced, purified by liquid chromatography (size exclusion and anions exchange) and characterized using 1H NMR and ESI-Q/TOF-MS. Finally, the production (in gram quantity) of pure unsaturated oligoglucuronans non-acetylated (di- and trisaccharide) was investigated thanks to the complete depolymerization of deacetylated glucuronan.

摘要

苜蓿中华根瘤菌M5N1CS合成一种由β-(1,4)连接的葡萄糖醛酸组成的均聚物，其C2和/或C3位存在可变的O-乙酰化修饰。为了获得β-Delta-(4,5)-不饱和低聚葡萄糖醛酸聚糖，该细菌聚合物的各种乙酰化形式被木霉属GL2葡萄糖醛酸聚糖裂解酶切割。然后制备了聚合度高达8的低聚物，通过液相色谱（尺寸排阻和阴离子交换）进行纯化，并使用1H NMR和ESI-Q/TOF-MS进行表征。最后，由于脱乙酰化葡萄糖醛酸聚糖的完全解聚，研究了纯的非乙酰化不饱和低聚葡萄糖醛酸聚糖（二糖和三糖）的克级产量。