里氏木霉编码属于新型多糖裂解酶家族的葡糖醛酸聚糖裂解酶的cDNA克隆。
Cloning of the Trichoderma reesei cDNA encoding a glucuronan lyase belonging to a novel polysaccharide lyase family.
作者信息
Konno Naotake, Igarashi Kiyohiko, Habu Naoto, Samejima Masahiro, Isogai Akira
机构信息
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
出版信息
Appl Environ Microbiol. 2009 Jan;75(1):101-7. doi: 10.1128/AEM.01749-08. Epub 2008 Oct 31.
Abstract
The filamentous fungus Trichoderma reesei produces glucuronan lyase (TrGL) when it is grown on beta-(1-->4)-polyglucuronate (cellouronate) as a sole carbon source. The cDNA encoding TrGL was cloned, and the recombinant enzyme was heterologously expressed in Pichia pastoris. The cDNA of TrGL includes a 777-bp open reading frame encoding a 20-amino-acid signal peptide and the 238-amino-acid mature protein. The amino acid sequence showed no similarity to the amino acid sequences of previously described functional proteins, indicating that the enzyme should be classified in a novel polysaccharide lyase (PL) family. Recombinant TrGL catalyzed depolymerization of cellouronate endolytically by beta-elimination and was highly specific for cellouronate. The enzyme was most active at pH 6.5 and 50 degrees C, and its activity and thermostability increased in the presence of Ca2+, suggesting that its calcium dependence is similar to that of other PLs, such as pectate lyases.
摘要
丝状真菌里氏木霉在以β-(1→4)-聚葡萄糖醛酸（纤维糖醛酸）作为唯一碳源生长时会产生葡萄糖醛酸裂解酶（TrGL）。编码TrGL的cDNA被克隆出来，重组酶在毕赤酵母中进行了异源表达。TrGL的cDNA包含一个777碱基对的开放阅读框，编码一个20个氨基酸的信号肽和238个氨基酸的成熟蛋白。该氨基酸序列与先前描述的功能蛋白的氨基酸序列没有相似性，这表明该酶应归类于一个新的多糖裂解酶（PL）家族。重组TrGL通过β-消除反应对内切纤维糖醛酸进行催化解聚，并且对纤维糖醛酸具有高度特异性。该酶在pH 6.5和50℃时活性最高，在Ca2+存在的情况下其活性和热稳定性会增加，这表明其对钙的依赖性与其他PLs（如果胶酸裂解酶）相似。
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本文引用的文献
1
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Nat Biotechnol. 2008 May;26(5):553-60. doi: 10.1038/nbt1403. Epub 2008 May 4.
2
Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium.担子菌黄孢原毛平革菌糖苷水解酶家族74木葡聚糖酶的底物识别
FEBS J. 2007 Nov;274(21):5727-36. doi: 10.1111/j.1742-4658.2007.06092.x. Epub 2007 Oct 8.
3
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Microbiology (Reading). 2006 Mar;152(Pt 3):617-625. doi: 10.1099/mic.0.28562-0.
4
Breakdown of oligosaccharides by the process of elimination.通过消除过程对寡糖进行分解。
Curr Opin Chem Biol. 2006 Apr;10(2):147-55. doi: 10.1016/j.cbpa.2006.02.005. Epub 2006 Feb 21.
5
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8
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