Suppr超能文献

里氏木霉编码属于新型多糖裂解酶家族的葡糖醛酸聚糖裂解酶的cDNA克隆。

Cloning of the Trichoderma reesei cDNA encoding a glucuronan lyase belonging to a novel polysaccharide lyase family.

作者信息

Konno Naotake, Igarashi Kiyohiko, Habu Naoto, Samejima Masahiro, Isogai Akira

机构信息

Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.

出版信息

Appl Environ Microbiol. 2009 Jan;75(1):101-7. doi: 10.1128/AEM.01749-08. Epub 2008 Oct 31.

DOI:10.1128/AEM.01749-08
PMID:18978091
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2612218/
Abstract

The filamentous fungus Trichoderma reesei produces glucuronan lyase (TrGL) when it is grown on beta-(1-->4)-polyglucuronate (cellouronate) as a sole carbon source. The cDNA encoding TrGL was cloned, and the recombinant enzyme was heterologously expressed in Pichia pastoris. The cDNA of TrGL includes a 777-bp open reading frame encoding a 20-amino-acid signal peptide and the 238-amino-acid mature protein. The amino acid sequence showed no similarity to the amino acid sequences of previously described functional proteins, indicating that the enzyme should be classified in a novel polysaccharide lyase (PL) family. Recombinant TrGL catalyzed depolymerization of cellouronate endolytically by beta-elimination and was highly specific for cellouronate. The enzyme was most active at pH 6.5 and 50 degrees C, and its activity and thermostability increased in the presence of Ca2+, suggesting that its calcium dependence is similar to that of other PLs, such as pectate lyases.

摘要

丝状真菌里氏木霉在以β-(1→4)-聚葡萄糖醛酸（纤维糖醛酸）作为唯一碳源生长时会产生葡萄糖醛酸裂解酶（TrGL）。编码TrGL的cDNA被克隆出来，重组酶在毕赤酵母中进行了异源表达。TrGL的cDNA包含一个777碱基对的开放阅读框，编码一个20个氨基酸的信号肽和238个氨基酸的成熟蛋白。该氨基酸序列与先前描述的功能蛋白的氨基酸序列没有相似性，这表明该酶应归类于一个新的多糖裂解酶（PL）家族。重组TrGL通过β-消除反应对内切纤维糖醛酸进行催化解聚，并且对纤维糖醛酸具有高度特异性。该酶在pH 6.5和50℃时活性最高，在Ca2+存在的情况下其活性和热稳定性会增加，这表明其对钙的依赖性与其他PLs（如果胶酸裂解酶）相似。

相似文献

1
Cloning of the Trichoderma reesei cDNA encoding a glucuronan lyase belonging to a novel polysaccharide lyase family.
Appl Environ Microbiol. 2009 Jan;75(1):101-7. doi: 10.1128/AEM.01749-08. Epub 2008 Oct 31.
2
Crystal structure of polysaccharide lyase family 20 endo-beta-1,4-glucuronan lyase from the filamentous fungus Trichoderma reesei.
FEBS Lett. 2009 Apr 17;583(8):1323-6. doi: 10.1016/j.febslet.2009.03.034. Epub 2009 Mar 22.
3
Efficient expression of an alkaline pectate lyase gene from Bacillus subtilis and the characterization of the recombinant protein.
Biotechnol Lett. 2012 Jan;34(1):109-15. doi: 10.1007/s10529-011-0734-1. Epub 2011 Sep 14.
4
Cloning, sequence analysis, and expression of gene alyPI encoding an alginate lyase from marine bacterium Pseudoalteromonas sp. CY24.
Can J Microbiol. 2009 Sep;55(9):1113-8. doi: 10.1139/w09-051.
5
A bacterial endo-β-1,4-glucuronan lyase, CUL-I from Brevundimonas sp. SH203, belonging to a novel polysaccharide lyase family.
Protein Expr Purif. 2020 Feb;166:105502. doi: 10.1016/j.pep.2019.105502. Epub 2019 Sep 20.
6
Heterologous expression of a Penicillium purpurogenum pectin lyase in Pichia pastoris and its characterization.
Fungal Biol. 2014 May-Jun;118(5-6):507-15. doi: 10.1016/j.funbio.2014.04.002. Epub 2014 Apr 13.
7
Purification and characterization of a novel glucuronan lyase from Trichoderma sp. GL2.
Appl Microbiol Biotechnol. 2006 Apr;70(4):437-43. doi: 10.1007/s00253-005-0077-8. Epub 2005 Jul 16.
8
Cloning of a gene encoding β-glucosidase from Chaetomium thermophilum CT2 and its expression in Pichia pastoris.
J Mol Microbiol Biotechnol. 2011;20(1):16-23. doi: 10.1159/000322606. Epub 2011 Jan 26.
9
Identification of a novel pelD gene expressed uniquely in planta by Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of its protein product as an endo-pectate lyase.
Arch Biochem Biophys. 1996 Aug 15;332(2):305-12. doi: 10.1006/abbi.1996.0346.
10
An Aspergillus oryzae acetyl xylan esterase: molecular cloning and characteristics of recombinant enzyme expressed in Pichia pastoris.
J Biotechnol. 2006 Feb 10;121(3):381-9. doi: 10.1016/j.jbiotec.2005.07.015. Epub 2005 Aug 29.

引用本文的文献

1
cazy_webscraper: local compilation and interrogation of comprehensive CAZyme datasets.
Microb Genom. 2023 Aug;9(8). doi: 10.1099/mgen.0.001086.
2
Description of the Wild Strain DSM26376, Reclassified under comb.nov., for Producing Glucuronan.
Polymers (Basel). 2023 May 3;15(9):2177. doi: 10.3390/polym15092177.
3
Discovery of a Novel Glucuronan Lyase System in .
Appl Environ Microbiol. 2022 Jan 11;88(1):e0181921. doi: 10.1128/AEM.01819-21. Epub 2021 Oct 27.
4
Cellulose induced protein 1 (Cip1) from Trichoderma reesei enhances the enzymatic hydrolysis of pretreated lignocellulose.
Microb Cell Fact. 2021 Jul 19;20(1):136. doi: 10.1186/s12934-021-01625-z.
5
Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction.
Sci Rep. 2020 Nov 18;10(1):20066. doi: 10.1038/s41598-020-77133-8.
6
Biochemical Characterization of a Bifunctional Enzyme Constructed by the Fusion of a Glucuronan Lyase and a Chitinase from sp.
Life (Basel). 2020 Oct 8;10(10):234. doi: 10.3390/life10100234.
7
Development of completely dispersed cellulose nanofibers.
Proc Jpn Acad Ser B Phys Biol Sci. 2018;94(4):161-179. doi: 10.2183/pjab.94.012.
8
Draft Genome Sequence of sp. Strain SH203, Producing Cellouronate (β-1,4-Linked Polyglucuronate) Lyase.
Genome Announc. 2017 May 4;5(18):e00262-17. doi: 10.1128/genomeA.00262-17.
9
Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase.
Biotechnol Biofuels. 2016 Feb 24;9:43. doi: 10.1186/s13068-016-0455-8. eCollection 2016.
10
The gentio-oligosaccharide gentiobiose functions in the modulation of bud dormancy in the herbaceous perennial Gentiana.
Plant Cell. 2014 Oct;26(10):3949-63. doi: 10.1105/tpc.114.131631. Epub 2014 Oct 17.

本文引用的文献

1
Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina).
Nat Biotechnol. 2008 May;26(5):553-60. doi: 10.1038/nbt1403. Epub 2008 May 4.
2
Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium.
FEBS J. 2007 Nov;274(21):5727-36. doi: 10.1111/j.1742-4658.2007.06092.x. Epub 2007 Oct 8.
3
Pectate lyase C from Bacillus subtilis: a novel endo-cleaving enzyme with activity on highly methylated pectin.
Microbiology (Reading). 2006 Mar;152(Pt 3):617-625. doi: 10.1099/mic.0.28562-0.
4
Breakdown of oligosaccharides by the process of elimination.
Curr Opin Chem Biol. 2006 Apr;10(2):147-55. doi: 10.1016/j.cbpa.2006.02.005. Epub 2006 Feb 21.
5
Purification and characterization of a novel glucuronan lyase from Trichoderma sp. GL2.
Appl Microbiol Biotechnol. 2006 Apr;70(4):437-43. doi: 10.1007/s00253-005-0077-8. Epub 2005 Jul 16.
6
Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism.
J Mol Biol. 2005 Jul 29;350(5):974-86. doi: 10.1016/j.jmb.2005.05.055.
7
Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp. strain.
J Biotechnol. 2005 Sep 10;118(4):448-57. doi: 10.1016/j.jbiotec.2005.04.014.
8
Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium.
FEBS J. 2005 Jun;272(11):2869-77. doi: 10.1111/j.1742-4658.2005.04707.x.
9
A twisted base? The role of arginine in enzyme-catalyzed proton abstractions.
Arch Biochem Biophys. 2005 Jan 1;433(1):266-78. doi: 10.1016/j.abb.2004.09.018.
10
Structure and function of a hypothetical Pseudomonas aeruginosa protein PA1167 classified into family PL-7: a novel alginate lyase with a beta-sandwich fold.
J Biol Chem. 2004 Jul 23;279(30):31863-72. doi: 10.1074/jbc.M402466200. Epub 2004 May 10.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验