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本文引用的文献

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Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina).生物质降解真菌里氏木霉（同义词：嗜热毁丝霉）的基因组测序与分析
Nat Biotechnol. 2008 May;26(5):553-60. doi: 10.1038/nbt1403. Epub 2008 May 4.
2
Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium.担子菌黄孢原毛平革菌糖苷水解酶家族74木葡聚糖酶的底物识别
FEBS J. 2007 Nov;274(21):5727-36. doi: 10.1111/j.1742-4658.2007.06092.x. Epub 2007 Oct 8.
3
Pectate lyase C from Bacillus subtilis: a novel endo-cleaving enzyme with activity on highly methylated pectin.来自枯草芽孢杆菌的果胶酸裂解酶C：一种对高度甲基化果胶具有活性的新型内切酶。
Microbiology (Reading). 2006 Mar;152(Pt 3):617-625. doi: 10.1099/mic.0.28562-0.
4
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Curr Opin Chem Biol. 2006 Apr;10(2):147-55. doi: 10.1016/j.cbpa.2006.02.005. Epub 2006 Feb 21.
5
Purification and characterization of a novel glucuronan lyase from Trichoderma sp. GL2.来自木霉属GL2菌株的一种新型葡糖醛酸聚糖裂解酶的纯化与特性分析
Appl Microbiol Biotechnol. 2006 Apr;70(4):437-43. doi: 10.1007/s00253-005-0077-8. Epub 2005 Jul 16.
6
Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism.芽孢杆菌属GL1黄原胶裂解酶与底物的晶体结构：对酶反应机制的见解
J Mol Biol. 2005 Jul 29;350(5):974-86. doi: 10.1016/j.jmb.2005.05.055.
7
Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp. strain.利用木霉菌株中的一种新的葡糖醛酸聚糖裂解酶活性生产葡糖醛酸寡糖。
J Biotechnol. 2005 Sep 10;118(4):448-57. doi: 10.1016/j.jbiotec.2005.04.014.
8
Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium.担子菌黄孢原毛平革菌胞外黄素细胞色素纤维二糖脱氢酶的电子传递链反应
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A twisted base? The role of arginine in enzyme-catalyzed proton abstractions.一个扭曲的碱基？精氨酸在酶催化质子提取中的作用。
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Structure and function of a hypothetical Pseudomonas aeruginosa protein PA1167 classified into family PL-7: a novel alginate lyase with a beta-sandwich fold.一种被归类于PL-7家族的假单胞菌铜绿假单胞菌蛋白PA1167的结构与功能：一种具有β-三明治折叠的新型藻酸盐裂解酶
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里氏木霉编码属于新型多糖裂解酶家族的葡糖醛酸聚糖裂解酶的cDNA克隆。

Cloning of the Trichoderma reesei cDNA encoding a glucuronan lyase belonging to a novel polysaccharide lyase family.

作者信息

Konno Naotake, Igarashi Kiyohiko, Habu Naoto, Samejima Masahiro, Isogai Akira

机构信息

Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.

出版信息

Appl Environ Microbiol. 2009 Jan;75(1):101-7. doi: 10.1128/AEM.01749-08. Epub 2008 Oct 31.

DOI:10.1128/AEM.01749-08
PMID:18978091
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2612218/
Abstract

The filamentous fungus Trichoderma reesei produces glucuronan lyase (TrGL) when it is grown on beta-(1-->4)-polyglucuronate (cellouronate) as a sole carbon source. The cDNA encoding TrGL was cloned, and the recombinant enzyme was heterologously expressed in Pichia pastoris. The cDNA of TrGL includes a 777-bp open reading frame encoding a 20-amino-acid signal peptide and the 238-amino-acid mature protein. The amino acid sequence showed no similarity to the amino acid sequences of previously described functional proteins, indicating that the enzyme should be classified in a novel polysaccharide lyase (PL) family. Recombinant TrGL catalyzed depolymerization of cellouronate endolytically by beta-elimination and was highly specific for cellouronate. The enzyme was most active at pH 6.5 and 50 degrees C, and its activity and thermostability increased in the presence of Ca2+, suggesting that its calcium dependence is similar to that of other PLs, such as pectate lyases.

摘要

丝状真菌里氏木霉在以β-(1→4)-聚葡萄糖醛酸（纤维糖醛酸）作为唯一碳源生长时会产生葡萄糖醛酸裂解酶（TrGL）。编码TrGL的cDNA被克隆出来，重组酶在毕赤酵母中进行了异源表达。TrGL的cDNA包含一个777碱基对的开放阅读框，编码一个20个氨基酸的信号肽和238个氨基酸的成熟蛋白。该氨基酸序列与先前描述的功能蛋白的氨基酸序列没有相似性，这表明该酶应归类于一个新的多糖裂解酶（PL）家族。重组TrGL通过β-消除反应对内切纤维糖醛酸进行催化解聚，并且对纤维糖醛酸具有高度特异性。该酶在pH 6.5和50℃时活性最高，在Ca2+存在的情况下其活性和热稳定性会增加，这表明其对钙的依赖性与其他PLs（如果胶酸裂解酶）相似。