对患有或未患有幽门螺杆菌相关性慢性胃炎患者的胃H⁺/K⁺-ATP酶活性进行直接测量。

Direct measurement of gastric H+/K+-ATPase activities in patients with or without Helicobacter pylori-associated chronic gastritis.

作者信息

Thong-Ngam Duangporn, Tangkijvanich Pisit, Sampatanukul Pichet, Prichakas Paungpayom, Mahachai Varocha, Tosukowong Piyaratana

机构信息

Department of Physiology, Faculty of Medicine, Chulalongkorn University Hospital, Bangkok 10330, Thailand.

出版信息

World J Gastroenterol. 2005 Jun 21;11(23):3514-7. doi: 10.3748/wjg.v11.i23.3514.

DOI:10.3748/wjg.v11.i23.3514

PMID:15962365

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4315951/

Abstract

AIM

The role of Helicobacter pylori (H pylori) infection in gastric acid secretion of patients with chronic gastritis remains controversial. This study was designed to elucidate the effect of H pylori on H+/K+-ATPase activities in gastric biopsy specimens.

METHODS

Eighty-two patients with chronic gastritis who had undergone upper endoscopy were included in this study. H pylori infection was confirmed by rapid urease test and histology. Gastric H+/K+-ATPase activities and serum gastrin concentrations were measured by an enzymatic method and radioimmunoassay, respectively. For those patients who received triple therapy for eradicating H pylori, changes in the activity of gastric H+/K+-ATPase and serum gastrin levels were also measured.

RESULTS

The mean gastric H+/K+-ATPase activity in H pylori-positive group (42 patients) was slightly higher than that in H pylori-negative group (29 patients) (169.65+/-52.9 and 161.38+/-43.85 nmol Pi/(mg.h), respectively, P=0.301). After eradication of H pylori, the gastric H+/K+-ATPase activities slightly decreased compared to prior therapy (165.03+/-59.50 and 158.42+/-38.93 nmol Pi/(mg.h), respectively, P=0.805). The mean basal gastrin concentration was slightly higher in H pylori-positive patients than in H pylori-negative patients (87.92+/-39.65 pg/mL vs 75.04+/-42.57 pg/mL, P=0.228). The gastrin levels fell significantly after the eradication of H pylori. (Before treatment 87.00+/-30.78 pg/mL, after treatment 64.73+/-18.96 pg/mL, P=0.015).

CONCLUSION

Gastric H+/K+-ATPase activities are not associated with H pylori status in patients with chronic gastritis.

摘要

目的

幽门螺杆菌（H pylori）感染在慢性胃炎患者胃酸分泌中的作用仍存在争议。本研究旨在阐明幽门螺杆菌对胃活检标本中H⁺/K⁺-ATP酶活性的影响。

方法

本研究纳入82例接受上消化道内镜检查的慢性胃炎患者。通过快速尿素酶试验和组织学检查确认幽门螺杆菌感染。分别采用酶法和放射免疫法测定胃H⁺/K⁺-ATP酶活性和血清胃泌素浓度。对于接受根除幽门螺杆菌三联疗法的患者，还测定了胃H⁺/K⁺-ATP酶活性和血清胃泌素水平的变化。

结果

幽门螺杆菌阳性组（42例患者）的平均胃H⁺/K⁺-ATP酶活性略高于幽门螺杆菌阴性组（29例患者）（分别为169.65±52.9和161.38±43.85 nmol Pi/（mg·h），P = 0.301）。根除幽门螺杆菌后，胃H⁺/K⁺-ATP酶活性与治疗前相比略有下降（分别为165.03±59.50和158.42±38.93 nmol Pi/（mg·h），P = 0.805）。幽门螺杆菌阳性患者的平均基础胃泌素浓度略高于幽门螺杆菌阴性患者（87.92±39.65 pg/mL对75.04±42.57 pg/mL，P = 0.228）。根除幽门螺杆菌后胃泌素水平显著下降。（治疗前87.00±30.78 pg/mL，治疗后64.73±18.96 pg/mL，P = 0.015）。