SNAP-23与L型钙通道之间的直接相互作用。
Direct interaction between SNAP-23 and L-type Ca2+ channel.
作者信息
Cho Won Jin, Jeremic Aleksandar, Jena Bhanu P
机构信息
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, 48201, USA.
出版信息
J Cell Mol Med. 2005 Apr-Jun;9(2):380-6. doi: 10.1111/j.1582-4934.2005.tb00363.x.
Abstract
During secretion, membrane-bound secretory vesicles dock and fuse at the base of porosomes in the cell plasma membrane. Among other proteins, the porosome is composed of SNAREs and Ca2+-channels. Ca2+-channels and SNAREs have been implicated in cell secretion. Several immunoprecipitation and binding studies suggest the physical interaction of the t-SNARE proteins, Syntaxin-1 and SNAP-25 with various Ca2+-channels. In this study, using yeast two-hybrid and immunoanalysis, we demonstrate for the first time, direct interaction of SNAP-23 and a L-type Ca2+-channel at the plasma membrane in pancreas.
摘要
在分泌过程中，膜结合的分泌囊泡在细胞质膜的孔体基部对接并融合。除其他蛋白质外，孔体由SNARE蛋白和Ca2+通道组成。Ca2+通道和SNARE蛋白与细胞分泌有关。多项免疫沉淀和结合研究表明，t-SNARE蛋白Syntaxin-1和SNAP-25与各种Ca2+通道存在物理相互作用。在本研究中，我们使用酵母双杂交和免疫分析，首次证明了胰腺质膜上SNAP-23与L型Ca2+通道的直接相互作用。
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