与附睾头、附睾体和附睾尾培养物共同孵育后公猪精子成熟度的评估[校正后]
Evaluation of boar sperm maturation after co-incubation with caput, corpus and cauda epididymal cultures [corrected].
作者信息
Bassols J, Kádár E, Briz M, Pinart E, Sancho S, Garcia-Gil N, Badia E, Pruneda A, Bussalleu E, Yeste M, Casas I, Dacheux J L, Bonet S
机构信息
Biotechnology of Porcine Reproduction, Department of Biology, INTEA, Faculty of Sciences, University of Girona, Campus Montilivi s/n, 17071 Girona, Spain.
出版信息
Theriogenology. 2005 Dec;64(9):1995-2009. doi: 10.1016/j.theriogenology.2005.05.027. Epub 2005 Jun 16.
Boar sperm from the proximal caput epididymis were co-incubated with 1, 4, 7, 10 and 14-day old caput, corpus and cauda epididymal cultures for 24, 48 and 72 h. Boar kidney epithelial cells (LLC-PK1) and ECM alone were used as negative controls. Sperm motility, morphology and membrane integrity were studied to evaluate boar sperm maturation in vitro. Our results showed that epithelial cell monolayers (10, 14-day old) create a suitable microenvironment for the survival of proximal caput sperm and the maintenance of sperm motility over a 72 h period. Moreover, corpus epididymal tubule fragments in culture (1, 4-day old) are capable of promoting the migration of the cytoplasmic droplet along the sperm tail after 24h of co-incubation.
将来自附睾头近端的公猪精子与1、4、7、10和14日龄的附睾头、附睾体和附睾尾培养物共同孵育24、48和72小时。单独使用公猪肾上皮细胞（LLC-PK1）和细胞外基质作为阴性对照。研究精子活力、形态和膜完整性以评估公猪精子的体外成熟。我们的结果表明，上皮细胞单层（10、14日龄）为附睾头近端精子的存活和72小时内精子活力的维持创造了合适的微环境。此外，培养的附睾体小管片段（1、4日龄）在共同孵育24小时后能够促进细胞质滴沿精子尾部迁移。
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