Sirivaidyapong S, Bevers M M, Gadella B M, Colenbrander B
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Yalelaan 7, 3584 CL Utrecht, The Netherlands.
Mol Reprod Dev. 2001 Apr;58(4):451-9. doi: 10.1002/1098-2795(20010401)58:4<451::AID-MRD14>3.0.CO;2-K.
In the current study we investigated the progesterone receptor exposure on the sperm from the testis and different parts of the epididymis, the relation to the sperm maturation stage, the functionality of the progesterone receptor and the capacity of sperm to undergo acrosome reaction. Exposed progesterone receptors on spermatozoa were detected using Progesterone-BSA conjugate labeled with fluorescein isothiocyanate (P-BSA-FITC) or a monoclonal antibody against progesterone receptor, C-262. Either progesterone or calcium ionophore was used to induce acrosome reaction. A high percentage (69 +/- 8%; mean +/- SD) of spermatozoa from the cauda epididymis showed P-BSA-FITC labeling at the onset of incubation, whereas only 0.1 +/- 1 and 4 +/- 2%, of spermatozoa from the testes, caput, and corpus epididymis, respectively, were labeled. There was no significant increase in P-BSA-FITC binding during the course of a 6 hr incubation. Treatment with either 10 microM progesterone or 5 microM calcium ionophore induced acrosome reaction in cauda epididymal sperm but not in testicular sperm, caput or corpus epipidymal sperm. It is concluded that the matured sperm of the dog from cauda epididymis and freshly ejaculated sperm demonstrate a functional membrane-bound progesterone receptor while less matured spermatozoa from the testicle, caput, and corpus epididymis fail to demonstrate such a receptor. Acrosome reaction of dog sperm can be induced using either progesterone or calcium ionophore; however, the maturation stages of spermatozoa influence this occurrence.
在本研究中，我们调查了来自睾丸和附睾不同部位的精子上孕酮受体的暴露情况、与精子成熟阶段的关系、孕酮受体的功能以及精子发生顶体反应的能力。使用异硫氰酸荧光素标记的孕酮-牛血清白蛋白偶联物（P-BSA-FITC）或抗孕酮受体的单克隆抗体C-262检测精子上暴露的孕酮受体。使用孕酮或钙离子载体诱导顶体反应。附睾尾部的精子在孵育开始时，有很高比例（69±8%；平均值±标准差）显示出P-BSA-FITC标记，而来自睾丸、附睾头和附睾体的精子分别只有0.1±1%和4±2%被标记。在6小时的孵育过程中，P-BSA-FITC结合没有显著增加。用10微摩尔孕酮或5微摩尔钙离子载体处理可诱导附睾尾部精子发生顶体反应，但不能诱导睾丸精子、附睾头或附睾体精子发生顶体反应。结论是，来自附睾尾部的成熟犬精子和新鲜射出的精子表现出功能性的膜结合孕酮受体，而来自睾丸、附睾头和附睾体的成熟度较低的精子则未表现出这种受体。犬精子的顶体反应可用孕酮或钙离子载体诱导；然而，精子的成熟阶段会影响这种反应的发生。
