Mikamo Eriko, Tanaka Chikako, Kanno Takashi, Akiyama Hideo, Jung Giman, Tanaka Hiroyuki, Kawai Tomoji
Toray Industries, Inc., New Frontiers Research Laboratories, 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan.
J Struct Biol. 2005 Jul;151(1):106-10. doi: 10.1016/j.jsb.2005.05.002.
The native polysomes of Saccharomyces cerevisiae were visualized in liquid solution by atomic force microscopy without external contrasting, such as shadowing and negative staining. This study showed native polysomes as lined particle with a height of ca. 27 nm, which is agreement with the height of 80S ribosomes in previous study. We found a small subparticle, located in a ring-shape or at the end of a linear structure, and visualized mRNA chains between adjacent ribosomes. Although the structures of polysomes have been studied for decades, it has remained difficult to visualize the native three-dimensional form. By the observation in liquid solution, we temporarily stopped the translation using an antibiotic to presenting the native three-dimensional structure and function of the polysomes. Our results provide not only new findings on native eukaryotic polysomes, but also great potential to visualize the influence of various environmental conditions on polysomes.
酿酒酵母的天然多聚核糖体在液体溶液中通过原子力显微镜观察到，无需诸如阴影和负染色等外部对比。这项研究显示天然多聚核糖体为高度约27 nm的排列颗粒，这与先前研究中80S核糖体的高度一致。我们发现了一个小亚基，位于环形或线性结构的末端，并观察到相邻核糖体之间的mRNA链。尽管多聚核糖体的结构已经研究了几十年，但仍然难以观察到其天然三维形态。通过在液体溶液中的观察，我们使用抗生素暂时停止翻译，以呈现多聚核糖体的天然三维结构和功能。我们的结果不仅提供了关于天然真核多聚核糖体的新发现，而且在观察各种环境条件对多聚核糖体的影响方面具有巨大潜力。
