Li Q-R, Ma J, Wang H, Li J-S
Research Institute of General Surgery, Nanjing General Hospital of Nanjing Command, Nanjing 210002, People's Republic of China.
Transplant Proc. 2005 Jun;37(5):2395-7. doi: 10.1016/j.transproceed.2005.03.115.
Acute allograft rejection is driven by production of cytokines such as interleukin(IL)-2 that activate and expand alloreactive T cells by ligating high-affinity IL-2alpha receptors. Lipid rafts on the membranes of T cells may be the functional microdomains for IL-2alpha receptor. Flow cytometric analysis was performed for expression of CD25 (IL-2alpha receptor) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2Ralpha in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence. Cells were stimulated with IL-2 and expression of CD25 (IL-2alpha receptor) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2Ralpha was localized in lipid rafts. Lipid rafts are the functional microdomains for IL-2alpha receptors.
急性同种异体移植排斥反应是由细胞因子如白细胞介素（IL）-2的产生所驱动的，这些细胞因子通过连接高亲和力的IL-2α受体来激活和扩增同种异体反应性T细胞。T细胞膜上的脂筏可能是IL-2α受体的功能微区。对T细胞表面CD25（IL-2α受体）的表达进行了流式细胞术分析。通过不连续蔗糖密度梯度超速离心分离脂筏。通过免疫印迹法确定从蔗糖密度梯度中分离的各组分中IL-2Rα的定位，并通过化学发光法进行检测。用IL-2刺激细胞，T细胞表面CD25（IL-2α受体）的表达为37.08%。对蔗糖梯度各组分的免疫印迹分析表明，大部分IL-2Rα定位于脂筏中。脂筏是IL-2α受体的功能微区。
