Wanders R J, Denis S, van Roermund C W, Jakobs C, ten Brink H J
Department of Clinical Biochemistry (FO-226), University of Amsterdam, Netherlands.
Biochim Biophys Acta. 1992 May 8;1125(3):274-9. doi: 10.1016/0005-2760(92)90056-2.
We have investigated the activation of pristanic acid to its CoA-ester in rat liver. The results show that peroxisomes, mitochondria as well as microsomes contain pristanoyl-CoA synthetase activity. On the basis of competition experiments and immunoprecipitation studies using antibodies raised against rat liver microsomal long-chain fatty acyl-CoA synthetase (EC 6.2.1.3) we conclude that pristanic acid is activated by the same enzyme which activates long-chain fatty acids, i.e., long-chain fatty acyl-CoA synthetase.
我们研究了大鼠肝脏中降植烷酸被激活生成其辅酶A酯的过程。结果表明，过氧化物酶体、线粒体以及微粒体均含有降植烷酰辅酶A合成酶活性。基于竞争实验以及使用针对大鼠肝脏微粒体长链脂肪酰辅酶A合成酶（EC 6.2.1.3）产生的抗体进行的免疫沉淀研究，我们得出结论：激活降植烷酸的酶与激活长链脂肪酸的酶相同，即长链脂肪酰辅酶A合成酶。
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