Raeder U, Broda P
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, PO Box 88, Manchester M60 1QD, UK.
EMBO J. 1986 Jun;5(6):1125-7. doi: 10.1002/j.1460-2075.1986.tb04336.x.
The white rot fungus Phanerochaete chrysosporium is an efficient degrader of lignin. Development of classical genetics in this organism would require several years of work, so an alternative strategy was devised. As in the mapping of human genetic diseases, the approach depends on the presence of interallelic restriction site polymorphism, but differs in following their meiotic segregation into the haploid state. Using a set of 20 cloned sequences as probes we were able to demonstrate meiotic segregation of 14 restriction site polymorphisms from fruiting of a single dikaryotic culture and obtained evidence of genetic linkage within two groups of three and two of two. The method could be used to obtain a genetic map of this fungus and should be applicable to genetic analysis of any other organism for which haploid material is available.
白腐真菌黄孢原毛平革菌是一种高效的木质素降解菌。在这种生物体中开展经典遗传学研究需要数年时间，因此设计了一种替代策略。如同人类遗传疾病的图谱绘制一样，该方法依赖于等位基因间限制性酶切位点多态性的存在，但不同之处在于追踪它们在减数分裂过程中分离进入单倍体状态的情况。我们使用一组20个克隆序列作为探针，能够证明从单个双核培养物的子实体中14个限制性酶切位点多态性的减数分裂分离，并获得了两组三个和两组两个之间存在遗传连锁的证据。该方法可用于绘制这种真菌的遗传图谱，并且应该适用于任何有单倍体材料可用的其他生物体的遗传分析。
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